Azuma M, Suzutani T, Sazaki K, Yoshida I, Sakuma T, Yoshida T
J Gen Virol. 1987 Mar;68 ( Pt 3):835-43. doi: 10.1099/0022-1317-68-3-835.
Mice inoculated intravenously with 10 to 100 micrograms trehalose-6,6'-dimycolate in an oil-in-water emulsion (TDM emulsion) acquired high resistance to intranasal infection by influenza virus at 7 to 14 days, but not at 1 day, after treatment. Mice inoculated with an oil-in-water emulsion without TDM (control emulsion) did not resist infection. The activity of the reticuloendothelial system of mice inoculated with TDM emulsion or control emulsion was greatly stimulated 1 day and 14 days after treatment. Interferon production in response to influenza virus was augmented in lung and serum of TDM emulsion-treated mice. The augmented interferon production was greatly diminished in the TDM emulsion-treated mice by treatment with anti-Thy-1.2 monoclonal antibody. Production of haemagglutination-inhibiting antibody in the TDM emulsion-treated or control emulsion-treated mice was higher than that in untreated mice, although no difference was observed between the TDM emulsion-treated and control emulsion-treated mice. On the other hand, TDM emulsion treatment of mice did not influence the appearance of antibody-producing cells, nor the activity of natural killer cells in the mice. The enhanced resistance of mice was diminished by inoculating anti-interferon-alpha/beta serum before influenza virus infection. No detectable interferon activity was observed in lung and blood of mice inoculated with anti-interferon-alpha/beta serum prior to influenza virus infection. These results suggest that the augmented early interferon production in T-lymphocytes of TDM emulsion-treated mice in response to influenza virus may play an important role in the enhanced resistance.
将10至100微克海藻糖-6,6'-二霉菌酸酯溶于水包油乳剂（TDM乳剂）中经静脉接种给小鼠，在治疗后7至14天，小鼠对流感病毒鼻内感染获得高度抗性，但在1天时未获得。接种不含TDM的水包油乳剂（对照乳剂）的小鼠不抵抗感染。接种TDM乳剂或对照乳剂的小鼠的网状内皮系统活性在治疗后1天和14天受到极大刺激。TDM乳剂处理的小鼠的肺和血清中对流感病毒产生的干扰素增加。在用抗Thy-1.2单克隆抗体处理的TDM乳剂处理的小鼠中，增加的干扰素产生大大减少。TDM乳剂处理或对照乳剂处理的小鼠中血凝抑制抗体的产生高于未处理的小鼠，尽管在TDM乳剂处理和对照乳剂处理的小鼠之间未观察到差异。另一方面，对小鼠进行TDM乳剂处理不影响抗体产生细胞的出现，也不影响小鼠中自然杀伤细胞的活性。在流感病毒感染前接种抗α/β干扰素血清可降低小鼠增强的抗性。在流感病毒感染前接种抗α/β干扰素血清的小鼠的肺和血液中未观察到可检测到的干扰素活性。这些结果表明，TDM乳剂处理的小鼠的T淋巴细胞中对流感病毒早期干扰素产生的增加可能在增强的抗性中起重要作用。
