Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Center for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
J Hazard Mater. 2022 Mar 15;426:128001. doi: 10.1016/j.jhazmat.2021.128001. Epub 2021 Dec 8.
The dissemination of antibiotic resistance genes (ARGs) increases risks towards human health and environmental safety. This work investigates the control of ARGs abundance and bacterial community evolution involved in waste activated sludge (WAS) treatment by chemical conditioning and subsequent mesophilic anaerobic digestion (MAD). The different chemical oxidation processes of ferrous iron-activated oxone and hydrogen peroxide (PMS-Fe and HO-Fe) and thermal-activated oxone (PMS@80 ℃) were investigated, and the ferric chloride (FeCl) and inactivated oxone (PMS) were compared. PMS@80 ℃ decreased the absolute abundance of most ARGs by 10.6-99.3% and that of total ARGs by 66.3%. Interestingly, oxidation pretreatment increased rather than decreased the relative abundance of most ARGs. MAD with PMS@80 ℃ pretreatment increased the absolute abundance of total ARGs by 51.6%, and other MAD processes decreased it by 8.6-47.4%. PMS-Fe and PMS@80 ℃ negatively inhibited methane production from 98.3 to 81.7 and 94.4 mL/g VSS in MAD. MAD effluent showed high abundance of Arcobacter genus in the range of 8.1-17.4% upon PMS-based pretreatment, possibly related to sulfur oxidation, nitrate reduction, and blaVEB enrichment. The radicals-orientated chemical oxidation can hardly improve the ARGs elimination by MAD due to the extremely high competitive organics in sludge.
抗生素耐药基因（ARGs）的传播增加了人类健康和环境安全的风险。本研究通过化学调理和随后的中温厌氧消化（MAD）来研究控制废活性污泥（WAS）处理过程中 ARGs 丰度和细菌群落演变的方法。研究了亚铁离子活化过氧单硫酸盐和过氧化氢（PMS-Fe 和 HO-Fe）以及热活化过氧单硫酸盐（PMS@80℃）的不同化学氧化过程，并比较了三氯化铁（FeCl）和失活过氧单硫酸盐（PMS）。PMS@80℃将大多数 ARGs 的绝对丰度降低了 10.6-99.3%，总 ARGs 的丰度降低了 66.3%。有趣的是，氧化预处理反而增加了大多数 ARGs 的相对丰度。用 PMS@80℃预处理的 MAD 使总 ARGs 的绝对丰度增加了 51.6%，而其他 MAD 过程则降低了 8.6-47.4%。PMS-Fe 和 PMS@80℃在 MAD 中分别从 98.3%和 94.4%抑制甲烷生成至 81.7%。在基于 PMS 的预处理后，MAD 废水中的 Arcobacter 属的丰度高达 8.1-17.4%，这可能与硫氧化、硝酸盐还原和 blaVEB 富集有关。由于污泥中存在极高的竞争有机物，自由基导向的化学氧化几乎无法通过 MAD 提高 ARGs 的去除率。
