Departamento de Medicina, Facultad de Salud, Universidad de Pamplona, Pamplona, Colombia.
Grupo de Virología, Dirección de Redes en Salud Pública, Instituto Nacional de Salud, Bogotá D.C., Colombia.
Biomedica. 2021 Dec 15;41(4):745-755. doi: 10.7705/biomedica.6032.
Enteric viruses have been associated with the production of a variety of diseases transmitted by the fecal-oral route and carried through contaminated food and water. Given their structure and composition, they are highly resistant to environmental conditions and most of the chemical agents used in the purification processes. Therefore, the systematic monitoring of raw water is necessary to ensure its quality especially when it is used for producing drinking water for human consumption.
We identified the presence of rotavirus and hepatitis A virus by means of the fluoro-immuno-magnetic separation technique (FIMS) in raw water taken from four purification plants and their water supplies in the department of Norte de Santander.
The viruses were captured and separated from the water samples using magnetic microparticles functionalized with monoclonal anti-Hepatitis A and anti-Rotavirus antibodies. Confocal microscopy was used to monitor the viral concentration process and transmission electron microscopy for the morphological visualization of the separated viruses. The reverse transcriptase-coupled polymerase chain reaction (RT-PCR) was applied to confirm the presence of pathogens.
The two enteric viruses were identified in the majority of the analyzed water samples including water supply sources.
We determined that the FIMS technique together with RT-PCR is highly effective for the detection of viral pathogens in complex matrices such as raw water.
肠病毒与通过粪-口途径传播的多种疾病有关，这些疾病可通过受污染的食物和水传播。由于其结构和组成，它们对环境条件和净化过程中使用的大多数化学剂具有很强的抵抗力。因此，有必要对原水进行系统监测，以确保其质量，特别是当原水用于生产供人类消费的饮用水时。
我们通过荧光免疫磁分离技术（FIMS）在北桑坦德省四个净化厂及其水源的原水中检测到轮状病毒和甲型肝炎病毒。
使用与单克隆抗甲型肝炎和抗轮状病毒抗体偶联的磁性微球从水样中捕获和分离病毒。共聚焦显微镜用于监测病毒浓缩过程，透射电子显微镜用于分离病毒的形态可视化。逆转录-聚合酶链反应（RT-PCR）用于确认病原体的存在。
在分析的大部分水样中，包括供水源，都发现了这两种肠病毒。
我们确定 FIMS 技术与 RT-PCR 相结合，非常适用于检测复杂基质（如原水）中的病毒病原体。
