Laboratory of Physiological Genetics, Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Science, Irkutsk 664033, Russia.
Laboratory of Medical and Biological Research, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, Ulan-Ude 670047, Russia.
Int J Med Mushrooms. 2021;23(11):37-44. doi: 10.1615/IntJMedMushrooms.2021040459.
This study examined effects of the styrylpyrone fraction from mycelium of hymenochaetoid species, Inonotus rheades (Agaricomycetes), on reactive oxygen species inhibition in Thellungiella salsuginea cell culture under oxidative stress. We identified antioxidant effects of styrylpyrones from I. rheades mycelium on oxidative stress in T. salsuginea cell suspension culture using two models of oxidative stress (induced by hyperthermia or hydrogen peroxide). The results showed that the styrylpyrone fraction maintained a high level of cell viability under stress conditions. The effect of pure hispidin on the plant cell culture was insignificant during the development of oxidative damage caused by hydrogen peroxide and was lacking during hyperthermia-induced oxidative stress. Therefore, these results suggest that the protective effect of the styrylpyrone fraction on T. salsuginea cells can be exerted by individual compounds (which are part of the fraction) and their complex.
本研究考察了担子果木层孔菌（伞菌纲）菌丝体的苯乙烯基吡啶酮部分对盐生衣藻细胞培养物在氧化应激下抑制活性氧的影响。我们使用两种氧化应激模型（由过热或过氧化氢诱导），鉴定了来自 I. rheades 菌丝体的苯乙烯基吡啶酮对 T. salsuginea 细胞悬浮培养物氧化应激的抗氧化作用。结果表明，在应激条件下，苯乙烯基吡啶酮部分保持了较高的细胞活力。在过氧化氢引起的氧化损伤和过热诱导的氧化应激过程中，纯希斯宾丁对植物细胞培养的影响并不显著。因此，这些结果表明，苯乙烯基吡啶酮部分对 T. salsuginea 细胞的保护作用可以通过其单体化合物（为该部分的一部分）及其复合物来发挥。
