School of Chemistry, University College Cork, Cork, Ireland.
Tyndall National Institute, Cork, Ireland.
Electrophoresis. 2022 Apr;43(7-8):857-864. doi: 10.1002/elps.202100347. Epub 2022 Jan 7.
Paracetamol (PAC) is one of the most extensively used analgesics and antipyretic drugs to treat mild and moderate pain. P-aminophenol (PAP), the main hydrolytic degradation product of PAC, can be found in environmental water. Recently, CE has been developed for the detection of a wide variety of chemical substances. The purpose of this study is to develop a simple and fast method for the detection and separation of PAC and its main hydrolysis product PAP using CE and microchip electrophoresis with capacitively coupled contactless conductivity detection. The determination of these compounds using microchip electrophoresis with capacitively coupled contactless conductivity detection is being reported for the first time. The separation was run for all analytes using a BGE (20 mM β-alanine, pH 11) containing 14% (v/v) methanol. The RSDs obtained for migration time were less than 4%, and RSDs obtained for peak area were less than 7%. The detection limits (S/N = 3) that were achieved ranged from 0.3 to 0.6 mg/L without sample preconcentration. The presented method showed rapid analysis time (less than 1 min), high efficiency and precision, low cost, and a significant decrease in the consumption of reagents. The microchip system has proved to be an excellent analytical technique for fast and reliable environmental applications.
对乙酰氨基酚（PAC）是一种广泛应用于治疗轻、中度疼痛的止痛和退热药物。对氨基酚（PAP）是 PAC 的主要水解降解产物，可在环境水中被发现。近年来，CE 已被开发用于检测各种化学物质。本研究旨在开发一种简单快速的 CE 和微芯片电泳与电容耦合非接触式电导检测联用检测和分离 PAC 及其主要水解产物 PAP 的方法。首次报道了使用微芯片电泳与电容耦合非接触式电导检测检测这些化合物。使用包含 14%（v/v）甲醇的 BGE（20 mM β-丙氨酸，pH 值为 11）对所有分析物进行分离。迁移时间的 RSD 小于 4%，峰面积的 RSD 小于 7%。无需样品预浓缩，检测限（S/N = 3）可达 0.3 至 0.6 mg/L。该方法具有分析时间短（不到 1 分钟）、效率高、精度高、成本低、试剂消耗显著减少等优点。微芯片系统已被证明是一种用于快速可靠的环境应用的出色分析技术。
