Linder J, Ye Y L, Harrington D S, Armitage J O, Weisenburger D D
Am J Pathol. 1987 Apr;127(1):1-8.
The authors investigated the ability of 70 monoclonal antibodies obtained from the Third International Workshop on Human Leukocyte Antigens (Oxford, 1986) to mark T lymphocytes in B5-fixed paraffin-embedded tissue. No staining occurred with 65 of the antibodies; however, 5 antibodies marked small lymphocytes in the T-cell areas of human tonsil. Two antibodies which strongly labeled lymphocytes, UCHL1 and T2/48, were used to examine 106 cases of non-Hodgkin's lymphoma, 29 cases of Hodgkin's disease, and a variety of normal and neoplastic tissues. UCHL1 and T2/48 each marked 86% (37/43) of B5-fixed T-cell lymphomas. Only 50% of formalin-fixed T-cell lymphomas were marked with these antibodies. UCHL1 marked 1.8% (1/56) of the B-cell lymphomas, compared with T2/48, which marked 19.6% (11/56) of the B-cell lymphomas. T2/48 had the interesting attribute of marking cells of the follicular mantle-zone and intermediate lymphocytic lymphoma, suggesting that the antibody recognizes a B-cell differentiation antigen. No Reed-Sternberg cells, epithelial neoplasms, sarcomas, neurogenic tumors, or normal nonlymphoid tissue were marked by either antibody. These antibodies successfully mark T cells in paraffin tissue sections and should aid in the investigation and characterization of abnormal lymphoid proliferations, "undifferentiated" malignant neoplasms, and immunologically mediated disorders.
作者研究了从第三届人类白细胞抗原国际研讨会（牛津，1986年）获得的70种单克隆抗体在B5固定的石蜡包埋组织中标记T淋巴细胞的能力。65种抗体未出现染色情况；然而，有5种抗体标记了人类扁桃体T细胞区域的小淋巴细胞。两种能强烈标记淋巴细胞的抗体UCHL1和T2/48，被用于检测106例非霍奇金淋巴瘤、29例霍奇金病以及多种正常和肿瘤组织。UCHL1和T2/48分别标记了86%（37/43）的B5固定的T细胞淋巴瘤。只有50%的福尔马林固定的T细胞淋巴瘤被这些抗体标记。UCHL1标记了1.8%（1/56）的B细胞淋巴瘤，而T2/48标记了19.6%（11/56）的B细胞淋巴瘤。T2/48具有标记滤泡套区细胞和中间淋巴细胞淋巴瘤的有趣特性，这表明该抗体识别一种B细胞分化抗原。两种抗体均未标记里德-斯腾伯格细胞、上皮性肿瘤、肉瘤、神经源性肿瘤或正常非淋巴组织。这些抗体成功地在石蜡组织切片中标记了T细胞，应有助于对异常淋巴增殖、“未分化”恶性肿瘤和免疫介导疾病的研究与特征描述。
