Abiko T, Sekino H
Biotechnol Appl Biochem. 1987 Feb;9(1):20-30.
A tritetracontapeptide corresponding to the entire amino acid sequence of endo-Arg38a-deacetylthymosin beta 10 was synthesized by a conventional solution method. Seven peptide fragments were assembled, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole-Me2Se in trifluoroacetic acid. In preliminary experiments the synthetic tritetracontapeptide increased the entire peripheral T-cell population and a helper T-cell subset when incubated in vitro with blood which was obtained from a uremic patient with pneumonia, but a suppressor/cytotoxic T-cell subset was unaffected under these conditions. The synthetic endo-Arg38a-deacetylthymosin beta 10 was as active as synthetic deacetylthymosin beta 10 in this in vitro assay.
通过传统溶液法合成了一种与内源性精氨酸38a - 脱乙酰胸腺素β10的完整氨基酸序列相对应的三十三肽。组装了七个肽片段，然后在三氟乙酸中用1 M三氟甲磺酸 - 苯甲硫醚 - 二甲基硒进行脱保护。在初步实验中，将合成的三十三肽与一名患有肺炎的尿毒症患者的血液在体外孵育时，可增加外周T细胞总数和辅助性T细胞亚群，但在这些条件下抑制性/细胞毒性T细胞亚群未受影响。在该体外试验中，合成的内源性精氨酸38a - 脱乙酰胸腺素β10与合成的脱乙酰胸腺素β10活性相同。
