Lin Cheng-Chih, Lin Hung-Yin, Thomas James L, Yu Jia-Xin, Lin Chien-Yu, Chang Yu-Hua, Lee Mei-Hwa, Wang Tzong-Liu
Division of Pulmonary Medicine, Department of Internal Medicine, Armed-Forces Zuoying General Hospital, Kaohsiung 81342, Taiwan.
Department of Electrical Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
Biomedicines. 2021 Dec 15;9(12):1923. doi: 10.3390/biomedicines9121923.
In this work, high-temperature pyrolysis was used to prepare both the core and shell of lantha-nide-doped UCNPs with lithium yttrium tetrafluoride (LiYF) to enhance the green luminescence. Merocyanine 540 (MC540)-grafted magnetic nanoparticles were incorporated in the PD-L1 pep-tide-imprinted poly(ethylene--vinyl alcohol) particles, which were formed by precipitation in a non-solvent. UCNPs in the non-solvent bath were thus entrapped in the imprinted particles to generate composite nanoparticles for the targeting and photodynamic therapy of PD-L1 in tumor cells. Finally, the in vitro cytotoxicity of the nanoparticles in HepG2 human liver cancer cells was evaluated with the continuous administration of MC540/MNPs@MIPs/UCNPs under irradiation by an NIR laser. To understand the delivery of the UCNP-embedded molecularly imprinted pol-ymers, the intrinsic and extrinsic pathways were also investigated.
在这项工作中，采用高温热解法制备了以四氟锂钇（LiYF）为基质的镧系掺杂上转换纳米粒子（UCNPs）的核与壳，以增强绿色发光。将接枝了部花青540（MC540）的磁性纳米粒子掺入通过在非溶剂中沉淀形成的程序性死亡配体1（PD-L1）肽印迹聚（乙烯-乙烯醇）颗粒中。非溶剂浴中的UCNPs因此被困在印迹颗粒中，以生成用于肿瘤细胞中PD-L1靶向和光动力治疗的复合纳米粒子。最后，通过在近红外激光照射下连续给药MC540/磁性纳米粒子@分子印迹聚合物/上转换纳米粒子，评估了纳米粒子对人肝癌HepG2细胞的体外细胞毒性。为了解嵌入上转换纳米粒子的分子印迹聚合物的递送情况，还研究了内在和外在途径。
