Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, China.
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, China.
Curr Microbiol. 2024 Oct 9;81(11):398. doi: 10.1007/s00284-024-03925-0.
Listeria monocytogenes, a zoonotic foodborne pathogen, presents a significant threat to global public health. Therefore, rapid and sensitive detection methods are crucial in mitigating the spread of L. monocytogenes induced diseases. This study introduced a loop-mediated isothermal amplification (LAMP) lyophilized powder detection reagent specifically designed for identifying Listeria monocytogenes. The reagent is user-friendly, quick, and can be easily transported and stored at room temperature. It exhibits no cross-reactivity with eight other types of bacteria and boasts a sensitivity of 10 CFU/mL. In a comparative study of 30 samples, the LAMP lyophilized powder detection reagent demonstrated higher sensitivity than the commercial Listeria monocytogenes qPCR detection kit. Additionally, the experimental time was reduced by approximately 30 min, making it highly suitable for rapid diagnosis. Preparation of lyophilized LAMP reagents may facilitate large-scale deployment, particularly in endemic areas or regions facing rapid outbreaks. This could greatly aid in controlling the transmission of pathogens, especially those transmitted through food.
单增李斯特菌是一种人畜共患病食源性致病菌，对全球公共卫生构成重大威胁。因此，快速、灵敏的检测方法对于减轻单增李斯特菌引发的疾病传播至关重要。本研究引入了一种环介导等温扩增（LAMP）冻干粉末检测试剂，专门用于鉴定单核细胞增生李斯特菌。该试剂使用方便、快速，可在室温下轻松运输和储存。它与其他八种细菌无交叉反应性，灵敏度为 10 CFU/mL。在对 30 个样本的比较研究中，LAMP 冻干粉末检测试剂的灵敏度高于商用单核细胞增生李斯特菌 qPCR 检测试剂盒。此外，实验时间缩短了约 30 分钟，非常适合快速诊断。制备冻干 LAMP 试剂可能有助于大规模部署，特别是在流行地区或面临快速爆发的地区。这将极大地有助于控制病原体的传播，尤其是通过食物传播的病原体。
