从……中分离出的四种三萜类化合物的抗炎活性。（原文不完整，此为根据现有内容的翻译）

Anti-Inflammatory Activity of Four Triterpenoids Isolated from .

作者信息

Zhang Lijia, Yin Mengzhou, Feng Xi, Ibrahim Salam A, Liu Ying, Huang Wen

机构信息

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Department of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USA.

出版信息

Foods. 2021 Dec 20;10(12):3155. doi: 10.3390/foods10123155.

In this study, triterpenoid compounds from were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-α, IL-1β, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 μg/mL. The results provide evidence for the use of as a natural anti-inflammatory agent in medicines and functional foods.

在本研究中，采用高速逆流色谱法（HSCCC）分离了[来源未提及]中的三萜类化合物，并使用超高效液相色谱四极杆飞行时间串联质谱（UHPLC-QTOF-MS/MS）和核磁共振（NMR）进行鉴定。还研究了纯化的三萜类化合物对RAW 264.7细胞的体外抗炎活性。获得了三萜类化合物、茯苓酸B、茯苓酸A、去氢土莫酸和去氢齿孔酸；它们的纯度分别为90%、92%、93%和96%。结果表明，茯苓酸B通过抑制脂多糖（LPS）诱导的RAW 264.7细胞中NO的生成，其抗炎活性高于茯苓酸A。然而，去氢土莫酸和去氢齿孔酸没有抗炎活性。此外，在10至40μg/mL的浓度范围内，用茯苓酸B处理的细胞中细胞因子（TNF-α、IL-1β和IL-6）的产生呈剂量依赖性降低。这些结果为[来源未提及]作为药物和功能性食品中的天然抗炎剂的应用提供了证据。