Li Qun, Guo Ailing, Ma Yi, Liu Ling, Liu Wukang, Zhong Yuan, Zhang Yawen
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070, China.
Hubei Provincial Institute for Food Supervision and Test, Wuhan 430070, China.
Microorganisms. 2021 Dec 15;9(12):2591. doi: 10.3390/microorganisms9122591.
is a zoonotic food-borne pathogen. The production of food-borne pathogenic bacteria aggregates is considered to be a way to improve their resistance and persistence in the food chain. has been shown to induce to form suspended aggregates, but induction mechanisms remain unclear. In the study, the effect of cell-free supernatants cultured in 10% TSB medium (10% RIS) on the formation of suspended aggregates was evaluated. Next, the Illumina RNA sequencing was used to compare the transcriptional profiles of in 10% TSB medium with and without 10% RIS to identify differentially expressed genes (DEGs). The result of functional annotation analysis of DEGs indicated that these genes mainly participate in two component system, bacterial chemotaxis and flagellar assembly. Then the reaction network of suspended aggregates with the presence of 10% RIS was summarized. The gene-deletion strain of was constructed by homologous recombination. The result showed that and are key genes in the formation of suspended aggregates. This research is the preliminary verification of suspended aggregates' RNA sequencing and is helpful to analyze the aggregation mechanisms of food-borne pathogenic bacteria from a new perspective.
是一种人畜共患的食源性病原体。食源性病原体细菌聚集体的产生被认为是提高其在食物链中的抗性和持久性的一种方式。已证明能诱导形成悬浮聚集体，但诱导机制仍不清楚。在该研究中，评估了在10%胰酪大豆肉汤培养基（10%RIS）中培养的无细胞上清液对悬浮聚集体形成的影响。接下来，使用Illumina RNA测序比较在含和不含10%RIS的10%胰酪大豆肉汤培养基中的转录谱，以鉴定差异表达基因（DEG）。对DEG的功能注释分析结果表明，这些基因主要参与双组分系统、细菌趋化性和鞭毛组装。然后总结了在存在10%RIS的情况下悬浮聚集体的反应网络。通过同源重组构建了的基因缺失菌株。结果表明和是形成悬浮聚集体的关键基因。本研究是对悬浮聚集体RNA测序的初步验证，有助于从新的角度分析食源性病原体的聚集机制。
