Department of Pneumology, "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, 8th Victor Babeș Street, 400012 Cluj, Romania.
"Leon Daniello" Clinical Hospital of Pneumology, 6th Bogdan Petriceicu Hașdeu Street, 400332 Cluj, Romania.
Medicina (Kaunas). 2021 Dec 10;57(12):1351. doi: 10.3390/medicina57121351.
: Chronic obstructive pulmonary disease (COPD) represents a debilitating disease, with rising morbidity and mortality. Vascular endothelial growth factor (VEGF) plays a major role in angiogenesis, vascular permeability, and airway remodeling. The purpose of this study was to investigate the relationship between VEGF serum levels and VEGF +936 C/T gene polymorphism (rs3025039) with COPD, for the first time in a Romanian population. : In total, 120 participants from Transylvania were included in this case-control study. Serum levels of VEGF were determined using an enzyme-linked immune-sorbent assay and rs3025039 was investigated by high molecular weight genomic deoxyribonucleic acid (DNA). Spirometric values, arterial blood gas analysis, and the Six Minute Walk Test (6MWT) outcome were also determined. : The serum level of VEGF was higher in the COPD group versus controls ( < 0.001), with a positive correlation with the 6MWT outcome. No significant difference was observed in the VEGF serum levels between VEGF +936C/T genotypes. There was no difference in the VEGF +936C/T genotype between COPD patients and healthy subjects (chi2 test = 0.92, OR = 1.04, 95%CI = 0.41-2.62), but the presence of the T allele was significantly linked to the presence of COPD (chi2 test = 0.02, OR = 2.36, 95%CI = 1.12-4.97). : Higher VEGF serum levels were found in moderate and severe COPD and were positively correlated with the distance in the 6MWT. No significant difference was found between CC, CT, and TT genotypes of rs3025039 and the presence of COPD. The presence of the T allele was found to be linked to COPD and also to the degree of airway obstruction.
: 慢性阻塞性肺疾病（COPD）是一种使人衰弱的疾病，发病率和死亡率不断上升。血管内皮生长因子（VEGF）在血管生成、血管通透性和气道重塑中起着重要作用。本研究旨在首次在罗马尼亚人群中研究 VEGF 血清水平与 VEGF+936C/T 基因多态性（rs3025039）与 COPD 之间的关系。: 在这项病例对照研究中，共纳入了来自特兰西瓦尼亚的 120 名参与者。使用酶联免疫吸附试验测定 VEGF 血清水平，通过高分子量基因组脱氧核糖核酸（DNA）研究 rs3025039。还测定了肺活量计值、动脉血气分析和六分钟步行试验（6MWT）结果。: COPD 组的 VEGF 血清水平高于对照组（<0.001），与 6MWT 结果呈正相关。VEGF+936C/T 基因型之间的 VEGF 血清水平没有差异。COPD 患者和健康受试者之间的 VEGF+936C/T 基因型没有差异（卡方检验=0.92，OR=1.04，95%CI=0.41-2.62），但 T 等位基因的存在与 COPD 的存在显著相关（卡方检验=0.02，OR=2.36，95%CI=1.12-4.97）。: 在中度和重度 COPD 中发现 VEGF 血清水平较高，与 6MWT 中的距离呈正相关。rs3025039 的 CC、CT 和 TT 基因型与 COPD 的存在之间没有发现显著差异。T 等位基因的存在与 COPD 以及气道阻塞程度有关。
