Wang Ming, Fu Ningning, Gao Chenglong, Wang Lixia, Ren Lili, Luo Youqing
Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Sino-French Joint Laboratory for Invasive Forest Pests in Eurasia, INRAE-Beijing Forestry University, Beijing 100083, China.
J Fungi (Basel). 2021 Dec 11;7(12):1065. doi: 10.3390/jof7121065.
along with its mutualistic fungal symbiont, (a white rot fungus), is an invasive pest that causes excessive damage to plantations in Northeast China. In 2015, were found to attack var. , and often share larval habitat with the native woodwasp, . The objective of this study was to determine the possible origin(s) of the introduced pest complex in China and analyse the genetic diversity between isolated from invasive , native and other woodwasps collected from Europe (native range) and other countries. Phylogenetic analyses were performed using the intergenic spacer (IGS) dataset and the combined 4-locus dataset (the internal transcribed spacer region (ITS), translation elongation factor alpha 1 (), DNA-directed ribosomal polymerase II (), and mitochondrial small subunit (mtSSU)) of three taxa. The multilocus genotyping of nuclear ribosomal regions and protein coding genes revealed at least three distinct multilocus genotypes (MLGs) of the fungus associated with invasive populations in Northeast China, which may have come from North America or Europe. The IGS region of carried by from China was designated type B1D2. Our results showed a lack of fidelity (the paradigm of obligate fidelity to a single fungus per wasp species) between woodwasp hosts and . We found that the native predominantly carried IGS-D2, but a low percentage of females instead carried IGS-B1D2 (MLG A13), which was presumably due to horizontal transmission from , during the sequential use of the same wood for larval development. The precise identification of the genotypes provides valuable insight into co-evolution between Siricidae and their symbionts, as well as understanding of the geographical origin and history of both species and their associated fungi.
与其共生的真菌共生体（一种白腐真菌）一起，是一种入侵性害虫，对中国东北地区的种植园造成了严重破坏。2015年，发现其攻击 变种，并且经常与本地树蜂 共享幼虫栖息地。本研究的目的是确定引入中国的害虫复合体的可能来源，并分析从入侵的 、本地的 以及从欧洲（原生范围）和其他国家收集的其他树蜂中分离出的 之间的遗传多样性。使用三个 类群的基因间隔区（IGS）数据集和组合的4个基因座数据集（内部转录间隔区（ITS）、翻译延伸因子α1（ ）、DNA指导的核糖体聚合酶II（ ）和线粒体小亚基（mtSSU））进行系统发育分析。核糖体区域和蛋白质编码基因的多位点基因分型揭示了与中国东北地区入侵 种群相关的真菌至少有三种不同的多位点基因型（MLGs），它们可能来自北美或欧洲。来自中国的 携带的 的IGS区域被指定为B1D2型。我们的结果表明，树蜂宿主和 之间缺乏专一性（每个树蜂物种对单一真菌专一性的范式）。我们发现本地 主要携带IGS-D2，但有一小部分雌性携带的是IGS-B1D2（MLG A13），这可能是由于在幼虫发育顺序使用同一木材期间，从 进行了水平传播。 基因型的精确鉴定为了解树蜂科与其共生体之间的共同进化，以及了解 物种及其相关真菌的地理起源和历史提供了有价值的见解。
