Wang Ming, Gao ChengLong, Xu QinWang, Fu NingNing, Li JiaLe, Ren LiLi, Luo YouQing
Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, China.
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, China.
Front Microbiol. 2024 Jul 11;15:1341646. doi: 10.3389/fmicb.2024.1341646. eCollection 2024.
In northeast China, the invasive woodwasp., , attacks var. Litv and often shares habitat with native . Previous research showed that can utilize the volatiles from its symbiotic fungus ( IGS-BD) to locate host trees. Consequently, symbiotic fungi ( IGS-D and ) carried by may influence the behavioral selection of . This study aimed to investigate the impact of fungal odor sources on 's behavior in laboratory and field experiments. Our observations revealed that female woodwasps exhibited greater attraction toward the fungal volatiles of 14-day-old IGS-D in a "Y"-tube olfactometer and wind tunnel. When woodwasps were released into bolts inoculated separately with three strains in the field, females of exhibited a preference for those bolts pre-inoculated with IGS-BD. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the volatiles emitted by the two genotypes of were similar yet significantly distinct from those of . Hence, we postulate that the existence of native IGS-D could potentially facilitate the colonization of in scenarios with minimal or no IGS-BD present in the host.
在中国东北地区，入侵性木蜂，攻击 变种Litv，并且常常与本地的 共享栖息地。先前的研究表明， 可以利用其共生真菌（IGS - BD）的挥发物来定位寄主树木。因此， 携带的共生真菌（IGS - D和 ）可能会影响 的行为选择。本研究旨在通过实验室和野外实验调查真菌气味源对 行为的影响。我们的观察结果显示，在“Y”型嗅觉仪和风洞中，雌性木蜂对14日龄的IGS - D真菌挥发物表现出更大的吸引力。当木蜂在野外被释放到分别接种了三种菌株的原木中时， 的雌性对预先接种了IGS - BD的原木表现出偏好。气相色谱 - 质谱联用（GC - MS）分析表明，两种基因型的 所释放的挥发物相似，但与 的挥发物有显著差异。因此，我们推测，在寄主中存在极少或不存在IGS - BD的情况下，本地IGS - D的存在可能会促进 的定殖。
