Li Li, Xu Na, Chen Fusheng
Hubei International Scientific and Technological Cooperation Base of Traditional Fermented Foods, Huazhong Agricultural University, Wuhan 430070, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
J Fungi (Basel). 2021 Dec 19;7(12):1094. doi: 10.3390/jof7121094.
pigments (MPs) have been used as food colorants for several centuries in Asian countries and are currently used around the world via Asian catering. The MPs biosynthetic pathway has been well-illustrated; however, the functions of a few genes including in the MPs gene cluster of M7 are still unclear. In the current study, was disrupted in ΔΔ, a highly efficient gene modification system, using as a selection marker, and ΔΔΔ:: and ΔΔΔ have been obtained. Subsequently, their morphologies, biomasses, MPs and citrinin (CIT) production were analyzed, respectively. These results have revealed that the deletion of has significant effects on the morphology and growth of M7. Moreover, compared with M7, the yields of MPs and CIT were drastically increased and decreased in mutants, respectively.
在亚洲国家，微生物色素（MPs）作为食品着色剂已使用了几个世纪，目前通过亚洲餐饮在全球范围内使用。MPs的生物合成途径已得到充分阐明；然而，包括M7的MPs基因簇中的几个基因的功能仍不清楚。在本研究中，使用[具体物质]作为选择标记，在高效基因修饰系统ΔΔ中破坏了[基因名称]，并获得了ΔΔΔ::[具体基因组合]和ΔΔΔ。随后，分别分析了它们的形态、生物量、MPs和桔霉素（CIT）的产生。这些结果表明，[基因名称]的缺失对M7的形态和生长有显著影响。此外，与M7相比，MPs突变体中MPs的产量大幅增加，而CIT的产量则大幅下降。
