Minagawa H, Takenaka A, Itoyama Y, Mori R
J Neurol Sci. 1987 Apr;78(2):225-35. doi: 10.1016/0022-510x(87)90063-3.
Relapse of experimental allergic encephalomyelitis (EAE) was achieved in Lewis rats by cyclophosphamide (CY). All rats, immunized with an emulsion of guinea pig spinal cord homogenate in complete Freund's adjuvant and treated with 100 mg/kg of CY 21 days postimmunization (pi), developed moderate to severe paralysis 9-14 days following CY injection. A second relapse was observed in 4 of 11 rats reinjected with CY 49 days pi. Histologically, focal mononuclear cell infiltration with or without demyelination of the white matter of the central nervous system was observed. Cyclophosphamide administration caused transient leukopenia and T-cell defect, the resolution of which coincided with relapse of clinical EAE. Lymphocyte proliferative responses to myelin basic protein (BP) and concanavalin A (Con A) and antibody titers to BP were preserved in CY-treated rats. Adoptive transfer of EAE to naive recipients with Con A-activated spleen cells from donors with CY-induced relapse was unsuccessful.
通过环磷酰胺（CY）使实验性变应性脑脊髓炎（EAE）在Lewis大鼠中复发。所有用豚鼠脊髓匀浆与完全弗氏佐剂的乳剂免疫且在免疫后21天（pi）用100mg/kg CY处理的大鼠，在注射CY后9 - 14天出现中度至重度麻痹。在免疫后49天再次注射CY的11只大鼠中有4只观察到第二次复发。组织学上，观察到中枢神经系统白质有局灶性单核细胞浸润，伴有或不伴有脱髓鞘。给予环磷酰胺导致短暂性白细胞减少和T细胞缺陷，其恢复与临床EAE的复发同时发生。在经CY处理的大鼠中，对髓鞘碱性蛋白（BP）和伴刀豆球蛋白A（Con A）的淋巴细胞增殖反应以及针对BP的抗体滴度得以保留。将CY诱导复发的供体的Con A激活的脾细胞将EAE过继转移给未致敏受体未成功。
