Yu Xing, Zhang Deteng, Liu Chang, Liu Zhaodi, Li Yujun, Zhao Qunzi, Gao Changyou, Wang Yong
Department of Thyroid Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
Front Bioeng Biotechnol. 2021 Dec 9;9:744230. doi: 10.3389/fbioe.2021.744230. eCollection 2021.
Severe traction injuries after stretch to peripheral nerves are common and challenging to repair. The nerve guidance conduits (NGCs) are promising in the regeneration and functional recovery after nerve injuries. To enhance the repair of severe nerve traction injuries, in this study KHIFSDDSSE (KHI) peptides were grafted on a porous and micropatterned poly(D,L-lactide-co-caprolactone) (PLCL) film (MPLCL), which was further loaded with a nerve growth factor (NGF). The adhesion number of Schwann cells (SCs), ratio of length/width (L/W), and percentage of elongated SCs were significantly higher in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group . The electromyography (EMG) and morphological changes of the nerve after severe traction injury were improved significantly in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group . Hence, the NGCs featured with both bioactive factors (KHI peptides and NGF) and physical topography (parallelly linear micropatterns) have synergistic effect on nerve reinnervation after severe traction injuries.
周围神经拉伸后的严重牵拉伤很常见，修复起来具有挑战性。神经引导导管（NGCs）在神经损伤后的再生和功能恢复方面很有前景。为了增强严重神经牵拉伤的修复效果，在本研究中，将KHIFSDDSSE（KHI）肽接枝到多孔且有微图案的聚（D，L-丙交酯-共-己内酯）（PLCL）薄膜（MPLCL）上，并进一步负载神经生长因子（NGF）。与PLCL组相比，MPLCL-肽组和MPLCL-肽-NGF组中雪旺细胞（SCs）的黏附数量、长宽比（L/W）以及伸长SCs的百分比均显著更高。与PLCL组相比，MPLCL-肽组和MPLCL-肽-NGF组中严重牵拉伤后神经的肌电图（EMG）和形态学变化得到显著改善。因此，具有生物活性因子（KHI肽和NGF）和物理形貌（平行线性微图案）的NGCs对严重牵拉伤后的神经再支配具有协同作用。
