MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, 310058, China.
Macromol Biosci. 2020 Mar;20(3):e1900382. doi: 10.1002/mabi.201900382. Epub 2020 Feb 14.
The feasible fabrication of nerve guidance conduits (NGCs) with good biological performance is important for translation in clinics. In this study, poly(d,l-lactide-co-caprolactone) (PLCL) films loaded with various amounts (wt; 5%, 15%, 25%) of methylcobalamin (MeCbl) are prepared, and are further rolled and sutured to obtain MeCbl-loaded NGCs. The MeCbl can be released in a sustainable manner up to 21 days. The proliferation and elongation of Schwann cells, and the proliferation of Neuro2a cells are enhanced on these MeCbl-loaded films. The MeCbl-loaded NGCs are implanted into rats to induce the regeneration of 10 mm amputated sciatic nerve defects, showing the ability to facilitate the recovery of motor and sensory function, and to promote myelination in peripheral nerve regeneration. In particular, the 15% MeCbl-loaded PLCL conduit exhibits the most satisfactory recovery of sciatic nerves in rats with the largest diameter and thickest myelinated fibers.
可实现的神经导管（NGC）的制造具有良好的生物性能，对于临床转化非常重要。在这项研究中，制备了负载不同量（wt；5％、15％、25％）甲钴胺（MeCbl）的聚（DL-丙交酯-共-己内酯）（PLCL）薄膜，并进一步卷绕和缝合以获得负载 MeCbl 的 NGC。MeCbl 可持续释放长达 21 天。施万细胞的增殖和伸长，以及 Neuro2a 细胞的增殖在这些负载 MeCbl 的薄膜上得到增强。将负载 MeCbl 的 NGC 植入大鼠体内，诱导 10mm 切断的坐骨神经缺损的再生，显示出促进运动和感觉功能恢复以及促进周围神经再生髓鞘形成的能力。特别是，负载 15％MeCbl 的 PLCL 导管在大鼠中表现出最令人满意的坐骨神经恢复，其直径最大，有髓神经纤维最厚。
