Department of Ophthalmology, Daping Hospital, Army Medical Center of PLA, Chongqing, China.
Int Ophthalmol. 2020 Apr;40(4):975-984. doi: 10.1007/s10792-019-01270-x. Epub 2020 Jan 10.
Nerve growth factor (NGF) and ciliary neurotrophic factor (CNTF) are well-known neurotrophic factors and widely used in the clinical treatment for its promotion effect on peripheral nerve regeneration. And they were also recommended for the acute paralytic strabismus treatment. However, whether the NGF and CNTF have protective effect for the extraocular muscles of acute paralytic strabismus patients is still poorly understood.
In this study, we want to evaluate the biological function of NGF and CNTF on the extraocular muscle cells and reveale the regulation mechanism behind it.
Firstly, the relative expression of ngf and cntf was assessed by quantitative real-time RT-PCR. Then, the influence of NGF and CNTF on the extraocular muscle cell proliferation was determined by CCK-8. The inflammatory response in muscle cells after NGF and CNTF treatment was evaluated by ELISA and ROS detection. In addition to this, the up-stream regulation of the ngf and cntf expression was also studied. The TargetScan was used for the predication of potential miRNAs targeting with ngf and cntf 30-UTR, which is soon confirmed by luciferase activity assay.
all the results in this research indicated that NGF and CNTF could promote the muscle cell proliferation and inhibit the inflammatory levels, then exert protective effect on the muscle cell function.
All the results in this research indicated that NGF and CNTF could promote the muscle cell proliferation and inhibit the inflammatory levels, then exert protective effect on the muscle cell function.
It was conceivable that let 7-5p was the up-stream regulator of ngf and cntf, and let 7-5p might serve as a potential molecular target for acute paralytic strabismus treatment.
神经生长因子（NGF）和睫状神经营养因子（CNTF）是众所周知的神经营养因子，因其能促进周围神经再生而被广泛应用于临床治疗。它们也被推荐用于急性麻痹性斜视的治疗。然而，NGF 和 CNTF 是否对急性麻痹性斜视患者的眼外肌有保护作用仍知之甚少。
本研究旨在评估 NGF 和 CNTF 对眼外肌细胞的生物学功能，并揭示其背后的调节机制。
首先，通过定量实时 RT-PCR 评估 ngf 和 cntf 的相对表达。然后，通过 CCK-8 测定 NGF 和 CNTF 对眼外肌细胞增殖的影响。通过 ELISA 和 ROS 检测评估 NGF 和 CNTF 处理后肌肉细胞的炎症反应。此外，还研究了 ngf 和 cntf 表达的上游调节。使用 TargetScan 预测潜在的与 ngf 和 cntf 3'UTR 靶向的 miRNAs，然后通过荧光素酶活性测定进行验证。
本研究的所有结果均表明，NGF 和 CNTF 可促进肌肉细胞增殖，抑制炎症水平，从而对肌肉细胞功能发挥保护作用。
可以推测 let 7-5p 是 ngf 和 cntf 的上游调节剂，let 7-5p 可能是急性麻痹性斜视治疗的潜在分子靶点。
