Department of Tea Science, Zhejiang University, Hangzhou 310058, China.
Department of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Nutrients. 2021 Dec 7;13(12):4379. doi: 10.3390/nu13124379.
Theaflavin-3,3'-digallate (TF3) is the most important theaflavin monomer in black tea. TF3 was proved to reduce blood glucose level in mice and rats. However, the elaborate anti-diabetic mechanism was not well elucidated. In this work, human hepatoma G2 (HepG2) cells and zebrafish () were used simultaneously to reveal anti-diabetic effect of TF3. The results showed that TF3 could effectively rise glucose absorption capacity in insulin-resistant HepG2 cells and regulate glucose level in diabetic zebrafish. The hypoglycemic effect was mediated through down-regulating phosphoenolpyruvate carboxykinase and up-regulating glucokinase. More importantly, TF3 could significantly improve β cells regeneration in diabetic zebrafish at low concentrations (5 μg/mL and 10 μg/mL), which meant TF3 had a strong anti-diabetic effect. Obviously, this work provided the potential benefit of TF3 on hypoglycemic effect, regulating glucose metabolism enzymes, and protecting β cells. TF3 might be a promising agent for combating diabetes.
茶黄素-3,3′-二没食子酸酯（TF3）是红茶中最重要的茶黄素单体。TF3 已被证明可降低小鼠和大鼠的血糖水平。然而，其详细的抗糖尿病机制尚未阐明。在这项工作中，同时使用人肝癌 G2（HepG2）细胞和斑马鱼（）来揭示 TF3 的抗糖尿病作用。结果表明，TF3 可有效提高胰岛素抵抗 HepG2 细胞的葡萄糖吸收能力，并调节糖尿病斑马鱼的血糖水平。降血糖作用是通过下调磷酸烯醇丙酮酸羧激酶和上调葡萄糖激酶来介导的。更重要的是，TF3 可在低浓度（5μg/mL 和 10μg/mL）下显著改善糖尿病斑马鱼中的β细胞再生，这意味着 TF3 具有很强的抗糖尿病作用。显然，这项工作提供了 TF3 对降低血糖、调节葡萄糖代谢酶和保护β细胞的潜在益处。TF3 可能是对抗糖尿病的一种有前途的药物。
