Institute of Home and Food Sciences, Government College University, Faisalabad, 38040, Pakistan.
National institute of Food Science and Technology, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan.
Lipids Health Dis. 2018 Mar 27;17(1):57. doi: 10.1186/s12944-018-0688-6.
In recent decade, Hyperlipidemia related disorders like obesity, hypercholesterolemia and diabetes are considered as the leading killers for mankind. Fundamental nexus between nutrition and health diverting the consumers focus towards plant based natural products as a remedy against various metabolic syndrome. Considering this, present study was conducted to explicate the role of black tea polyphenols such as Theaflavins and thearubigins therapeutic potential to tackle targeted maladies especially oxidative stress related disorders like hypercholesterolemia and diabetes.
The mandate of current investigation was to explore the hypoglycemic and hypocholestrolemic perspective of isolated theaflavin and thearubigins through a model feeding trial. For the purpose, theaflavin & thearubigins were isolated from black tea through solvent partition method and utilize to form three types of nutraceutical drinks (theaflavin, thearubigins & theaflavin + thearubigins based) alongside control to be further utilized in bioefficacy trial. In bioefficacy trial, three types of independent studies were design on the bases of diet by involving 20 male wistar rats in each study (5 for each group). In study I, normal diet was administrated while, in study II & III high cholesterol and high sucrose diet was given, respectively along with prepared nutraceutical drinks to synchronize their therapeutic effect for a period of 56 days. At the termination of trial, Feed & drink intakes, body weight, total cholesterol, LDL, HDL, triglycerides, glucose and insulin levels were measured.
The results indicated reduction in cholesterol, LDL and triglycerides levels of experimental rats in all studies with significant increase in HDL. In this context, theaflavin based drink imparted maximum reduction in cholesterol (3.75, 11.03 & 10.39%), LDL (3.84, 14.25& 10.84%) & triglycerides (2.99, 8.54 & 6.65%) in respective studies compared to thearubigins and theaflavin + thearubigins based drinks. However, theaflavin+ thearubigins based drink caused highest glucose decline and maximum insulin increase in all studies as compared to other nutraceutical drinks. The reported value for the insulin increase were 13.02 ± 1.02 & 14.55 ± 1.13, 10.09 ± 0.15 & 11.59 ± 0.86 for Hyperglycemic and Hypocholestrolemic rats respectively compared to control (7.84 ± 0.45 & 9.10 ± 0.41) for study I and II.
In the nutshell, theaflavin and thearubigins based dietary interventions are helpful to alleviate the hypercholestrolemia and hyperglycemia and should be promoted as parallel therapy to combat these disorders.
在最近的十年中，肥胖、高胆固醇血症和糖尿病等与高血脂相关的疾病被认为是人类的主要杀手。营养与健康之间的基本联系促使消费者将注意力转向植物性天然产品，作为治疗各种代谢综合征的方法。有鉴于此，本研究旨在阐述红茶多酚（如茶黄素和茶红素）的作用，以及其作为治疗各种疾病的潜在疗法，特别是与氧化应激相关的疾病，如高胆固醇血症和糖尿病。
目前研究的任务是通过模型喂养试验探索茶黄素和茶红素的降血糖和降胆固醇作用。为此，通过溶剂分配法从红茶中分离出茶黄素和茶红素，并将其用于形成三种类型的营养饮料（茶黄素、茶红素和茶黄素+茶红素基），同时作为对照，进一步用于生物功效试验。在生物功效试验中，基于饮食设计了三种独立的研究，每个研究涉及 20 只雄性 Wistar 大鼠（每组 5 只）。在研究 I 中，给予正常饮食，而在研究 II 和研究 III 中，分别给予高胆固醇和高蔗糖饮食，并给予准备好的营养饮料，以同步它们的治疗效果，为期 56 天。在试验结束时，测量饲料和饮料摄入量、体重、总胆固醇、LDL、HDL、甘油三酯、血糖和胰岛素水平。
结果表明，所有研究中的实验大鼠的胆固醇、LDL 和甘油三酯水平均降低，HDL 水平显著升高。在这方面，与茶红素和茶黄素+茶红素基饮料相比，茶黄素基饮料在各自的研究中对胆固醇（3.75、11.03 和 10.39%）、LDL（3.84、14.25 和 10.84%）和甘油三酯（2.99、8.54 和 6.65%）的降低效果最大。然而，与其他营养饮料相比，茶黄素+茶红素基饮料在所有研究中均导致最高的血糖下降和最大的胰岛素增加。报告的胰岛素增加值分别为：研究 I 和 II 中高血糖和高胆固醇血症大鼠的 13.02±1.02 和 14.55±1.13、10.09±0.15 和 11.59±0.86，与对照组（7.84±0.45 和 9.10±0.41）相比。
总之，基于茶黄素和茶红素的饮食干预有助于缓解高胆固醇血症和高血糖症，应作为治疗这些疾病的辅助疗法加以推广。
