State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China.
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
Viruses. 2021 Dec 9;13(12):2464. doi: 10.3390/v13122464.
Viral pathogens are a major threat to stable crop production. Using a backcross strategy, we find that integrating a dominant brown planthopper (BPH) resistance gene into a high-yield and BPH-susceptible rice variety significantly enhances BPH resistance. However, when -carrying backcross lines are infested with BPH, these BPH-resistant lines exhibit sterile characteristics, displaying panicle enclosure and failure of seed production at their mature stage. As we suspected, BPH-mediated viral infections could cause the observed sterile symptoms, and we characterized rice-infecting viruses using deep metatranscriptomic sequencing. Our analyses revealed eight novel virus species and five known viruses, including a highly divergent virus clustered within a currently unclassified family. Additionally, we characterized rice plant antiviral responses using small RNA sequencing. The results revealed abundant virus-derived small interfering RNAs in sterile rice plants, providing evidence for Dicer-like and Argonaute-mediated immune responses in rice plants. Together, our results provide insights into the diversity of viruses in rice plants, and our findings suggest that multiple virus infections occur in rice plants.
病毒病原体是稳定作物生产的主要威胁。我们使用回交策略发现，将一个显性褐飞虱（BPH）抗性基因整合到一个高产和易感 BPH 的水稻品种中，显著增强了 BPH 抗性。然而，当携带 - 的回交系受到 BPH 侵害时，这些抗 BPH 的系表现出不育特征，在成熟阶段出现穗包封和种子生产失败。正如我们所怀疑的那样，BPH 介导的病毒感染可能导致观察到的不育症状，我们使用深度宏转录组测序来描述感染水稻的病毒。我们的分析揭示了八种新的病毒物种和五种已知的病毒，包括一种高度分化的病毒，聚类在一个目前未分类的家族中。此外，我们还使用小 RNA 测序来描述水稻的抗病毒反应。结果表明，在不育水稻植物中存在丰富的病毒衍生小干扰 RNA，为水稻植物中 Dicer-like 和 Argonaute 介导的免疫反应提供了证据。总之，我们的结果提供了对水稻植物中病毒多样性的深入了解，我们的研究结果表明，水稻植物中存在多种病毒感染。
