Zaytseva Anna, Chekanov Konstantin, Zaytsev Petr, Bakhareva Daria, Gorelova Olga, Kochkin Dmitry, Lobakova Elena
Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University, 1-12 Leninskie Gory, 119192 Moscow, Russia.
Centre for Humanities Research and Technology, National Research Nuclear University MEPhI, 31 Kashirskoye Highway, 115522 Moscow, Russia.
Plants (Basel). 2021 Nov 26;10(12):2601. doi: 10.3390/plants10122601.
The microalga dwells in habitats with excessive solar irradiation; consequently, it must accumulate diverse compounds to protect itself. We characterized the array of photoprotective compounds in . Toward this goal, we exposed the cells to high fluxes of visible light and UV-A and analyzed the ability of hydrophilic and hydrophobic extracts from the cells to absorb radiation. Potential light-screening compounds were profiled by thin layer chromatography and UPLC-MS. accumulated diverse carotenoids that absorbed visible light in the blue-green part of the spectrum and mycosporine-like amino acids (MAA) that absorbed the UV-A. It is the first report on the occurrence of MAA in . Two new MAA, named coelastrin A and coelastrin B, were identified. Transmission electron microscopy revealed the development of hydrophobic subcompartments under the high light and UV-A exposition. We also evaluate and discuss sporopollenin-like compounds in the cell wall and autophagy-like processes as the possible reason for the decrease in sunlight absorption by cells, in addition to inducible sunscreen accumulation. The results suggested that NAMSU R1 accumulates a broad range of valuable photoprotective compounds in response to UV-A and visible light irradiation, which indicates this strain as a potential producer for biotechnology.
这种微藻生活在阳光照射过度的环境中；因此，它必须积累多种化合物来保护自己。我们对……中的一系列光保护化合物进行了表征。为了实现这一目标，我们将细胞暴露在高通量的可见光和UV-A下，并分析了细胞亲水和疏水提取物吸收辐射的能力。通过薄层色谱和超高效液相色谱-质谱联用对潜在的光屏蔽化合物进行了分析。……积累了多种类胡萝卜素，它们在光谱的蓝绿色部分吸收可见光，还积累了吸收UV-A的类菌孢素氨基酸（MAA）。这是关于……中出现MAA的首次报道。鉴定出了两种新的MAA，命名为腔孢菌素A和腔孢菌素B。透射电子显微镜显示在高光和UV-A照射下疏水亚细胞区室的形成。除了可诱导的防晒剂积累外，我们还评估并讨论了细胞壁中类孢粉素样化合物和自噬样过程，它们可能是细胞阳光吸收减少的原因。结果表明，NAMSU R1在UV-A和可见光照射下积累了广泛的有价值的光保护化合物，这表明该菌株是生物技术的潜在生产者。
