Lee Youngsuk, Do Van Giap, Kim Seonae, Kweon Hunjoong
Apple Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration, 107, Soboangye-ro, Gunwi 39000, Korea.
Plants (Basel). 2021 Dec 2;10(12):2649. doi: 10.3390/plants10122649.
Nitrogen (N) is an essential macronutrient that regulates diverse physiological processes for plant survival and development. In apple orchards, inappropriate N conditions can cause imbalanced growth and subsequent physiological disorders in trees. In order to investigate the molecular basis underlying the physiological signals for N stress responses, we examined the metabolic signals responsive to contrasting N stress conditions (deficient/excessive) in apple leaves using transcriptome approaches. The clustering of differentially expressed genes (DEGs) showed the expression dynamics of genes associated with each N stress group. Functional analyses of gene ontology and pathway enrichments revealed the potential candidates of metabolic signals responsible for N-deficient/excessive stress responses. The functional interactions of DEGs in each cluster were further explored by protein-protein interaction network analysis. Our results provided a comprehensive insight into molecular signals responsive to N stress conditions, and will be useful in future research to enhance the nutrition tolerance of tree crops.
氮（N）是一种必需的大量营养素，它调节着植物生存和发育的多种生理过程。在苹果园中，不合适的氮条件会导致树木生长失衡并引发后续的生理紊乱。为了探究氮胁迫响应生理信号的分子基础，我们采用转录组方法研究了苹果叶片中对不同氮胁迫条件（缺乏/过量）作出响应的代谢信号。差异表达基因（DEG）的聚类显示了与每个氮胁迫组相关基因的表达动态。基因本体和通路富集的功能分析揭示了负责缺氮/过量胁迫响应的代谢信号的潜在候选基因。通过蛋白质-蛋白质相互作用网络分析进一步探索了每个聚类中DEG的功能相互作用。我们的结果为响应氮胁迫条件的分子信号提供了全面的见解，并将有助于未来提高果树营养耐受性的研究。
