Phanomchai Saowaros, Noichinda Sompoch, Kachonpadungkitti Yongsak, Bodhipadma Kitti
Division of Agro-Industrial Technology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand.
Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Khlong Luang, Pathum Thani 12121, Thailand.
Plants (Basel). 2021 Dec 4;10(12):2671. doi: 10.3390/plants10122671.
Persian violet flowers are considered esthetically attractive, leading to the high economic value of this plant. Plant breeding is fundamental to crop improvement, and the induction of mutation by tissue culture technology in combination with irradiation has been beneficially applied to generate plants with novel desirable characteristics. In this research, single or double rounds of UV-C irradiations were carried out on plant tissue cultures to initiate the in vitro rooting and mutation of Persian violets. It was found that single low-intensity UV-C exposure, when applied to Persian violet microshoots for 4 h, could induce the maximum number of roots and the highest root length without the use of a plant growth regulator. Overall, the single and double UV-C irradiation of Persian violet microshoots led to 44 different types of Persian violet flower mutations. Under single high-intensity UV-C irradiation for 6 h, up to nine petals were initiated, whereas single low-intensity UV-C irradiation did not influence the morphological variation of Persian violet flowers. Thus, Persian violet microshoots respond differently in terms of in vitro rooting and flowering depending on the UV-C intensity and exposure duration. These outcomes may be applied to micropropagation and in vitro plant breeding.
波斯紫罗兰花朵在美学上具有吸引力，这使得这种植物具有很高的经济价值。植物育种是作物改良的基础，通过组织培养技术结合辐射诱导突变已被有益地应用于培育具有新的理想特性的植物。在本研究中，对植物组织培养物进行了单轮或双轮紫外线-C照射，以启动波斯紫罗兰的离体生根和突变。研究发现，当对波斯紫罗兰微枝进行4小时的单轮低强度紫外线-C照射时，在不使用植物生长调节剂的情况下，可诱导出最多数量的根和最长的根长。总体而言，对波斯紫罗兰微枝进行单轮和双轮紫外线-C照射导致了44种不同类型的波斯紫罗兰花朵突变。在6小时的单轮高强度紫外线-C照射下，最多可诱导出9片花瓣，而单轮低强度紫外线-C照射对波斯紫罗兰花朵的形态变异没有影响。因此，波斯紫罗兰微枝在离体生根和开花方面的反应因紫外线-C强度和照射持续时间而异。这些结果可应用于微繁殖和离体植物育种。
