Cetin Emine Sema
Biol Res. 2014 Sep 4;47(1):37. doi: 10.1186/0717-6287-47-37.
The aim of the present work was to examine the role of UV-C irradiation on the production of secondary metabolites (total phenolic, total flavanols, total flavonols, catechin, ferulic acid and trans-resveratrol in phenolic compounds and α-, β-, γ- δ-tocopherols) in callus cultures. Studies on the effects of UV-C treatment on callus culture are seldom and generally focused on UV-B. However UV-C radiation play an important role in accumule secondary metabolites.
In this study, callus cultures from Öküzgözü grape cultivar were initiated from leaf petiole explants. Calli formed after 6 weeks on the medium supplemented with 0.5 mg L-1 benzylaminopurine (BA), 0.5 mg L-1 indole acetic acid (IAA) on B5 media. Callus tissues were exposed to UV-C irradiation at 10, 20 and 30 cm distances from the UV source for 5 and 10 minutes and samples were collected at hours 0, 24 and 48.
The greatest total phenolic content (155.14 mg 100 g-1) was detected in calli exposed to UV-C for 5 min from 30 cm distance and sampled after 24 h. 24 h and 48 h incubation times, 30 cm and 5 min were the most appropriate combination of UV-C application in total flavanol content. Maximum total flavonol content (7.12 mg 100 g-1) was obtained on 0 h, 5 min and 20 cm combination. The highest (+)- catechin accumulation (8.89 mg g-1) was found in calli with 10 min UV-C application from 30 cm distance and sampled after 48 h. Ferulic acid content increased 6 fold in Öküzgözü callus cultures (31.37 μg g-1) compared to the control group. The greatest trans-resveratrol content (8.43 μg g-1) was detected in calli exposed to UV-C for 5 min from 30 cm distance and sampled after 24 h. The highest α-tocopherol concentration was found in calli exposed to UV-C for 10 min from 30 cm distance and sampled after 24 h. As a conclusion, it was showed that UV-C radiation had remarkable promoting effects on the accumulation of secondary metabolites in the calli of Öküzgözü grape cultivar.
本研究的目的是考察紫外线C（UV-C）照射对愈伤组织培养物中次生代谢产物（酚类化合物中的总酚、总黄烷醇、总黄酮醇、儿茶素、阿魏酸和反式白藜芦醇以及α-、β-、γ-、δ-生育酚）生成的作用。关于UV-C处理对愈伤组织培养影响的研究很少，且通常集中在UV-B上。然而，UV-C辐射在次生代谢产物积累中发挥着重要作用。
在本研究中，从奥库兹戈祖葡萄品种的叶柄外植体诱导出愈伤组织培养物。在添加了0.5 mg L-1苄氨基嘌呤（BA）、0.5 mg L-1吲哚乙酸（IAA）的B5培养基上，6周后形成愈伤组织。将愈伤组织在距离紫外线源10、20和30 cm处分别照射UV-C 5分钟和10分钟，并在0、24和48小时采集样品。
在距离紫外线源30 cm处照射UV-C 5分钟并在24小时后取样的愈伤组织中，检测到最高的总酚含量（155.14 mg 100 g-1）。在总黄烷醇含量方面，24小时和48小时的培养时间、30 cm的照射距离和5分钟的照射时间是UV-C处理的最合适组合。在0小时、5分钟和20 cm的组合下，获得了最高的总黄酮醇含量（7.12 mg 100 g-1）。在距离紫外线源30 cm处照射UV-C 10分钟并在48小时后取样的愈伤组织中，发现了最高的（+）-儿茶素积累量（8.89 mg g-1）。与对照组相比，奥库兹戈祖葡萄愈伤组织培养物中的阿魏酸含量增加了6倍（31.37 μg g-1）。在距离紫外线源30 cm处照射UV-C 5分钟并在24小时后取样的愈伤组织中，检测到最高的反式白藜芦醇含量（8.43 μg g-1）。在距离紫外线源30 cm处照射UV-C 10分钟并在24小时后取样的愈伤组织中，发现了最高的α-生育酚浓度。总之，结果表明UV-C辐射对奥库兹戈祖葡萄品种愈伤组织中次生代谢产物的积累具有显著的促进作用。
