Zhao Jialin, Zhao Fu, Li Haolin, Xiong Youlin, Cai Shuangfei, Wang Chen, Chen Yunfa, Han Ning, Yang Rong
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, No.11 ZhongGuanCun BeiYiTiao, Beijing 100190, China.
Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100049, China.
Electrochim Acta. 2022 Feb 1;404:139766. doi: 10.1016/j.electacta.2021.139766. Epub 2021 Dec 23.
Tracking and monitoring of low concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can effectively control asymptomatic transmission of current coronavirus disease 2019 (COVID-19) in the early stages of infection. Here, we highlight an electrochemical immunosensor for sensitive detection of SARS-CoV-2 antigen marker spike protein. The surface-clean Pd-Au nanosheets as a substrate for efficient sensing and signal output have been synthesized. The morphology, chemical states and excellent stable electrochemical properties of this surface-clean heterostructures have been studied. Functionalized superparamagnetic nanoparticles (MNPs) were introduced as sample separators and signal amplifiers. This biosensor was tested in phosphate buffered saline (PBS) and nasopharyngeal samples. The results showed that the sensor has a wide linear dynamic range (0.01 ng mL to 1000 ng mL) with a low detection limit (0.0072 ng mL), which achieved stable and sensitive detection of the spike protein. Therefore, this immunosensing method provides a promising electrochemical measurement tool, which can furnish ideas for early screening and the reasonable optimization of detection methods of SARS-CoV-2.
追踪和监测低浓度的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）能够在感染的早期阶段有效控制当前2019冠状病毒病（COVID-19）的无症状传播。在此，我们重点介绍一种用于灵敏检测SARS-CoV-2抗原标志物刺突蛋白的电化学免疫传感器。已合成表面清洁的钯-金纳米片作为高效传感和信号输出的基底。已研究了这种表面清洁异质结构的形貌、化学状态和优异的稳定电化学性质。引入功能化超顺磁性纳米颗粒（MNPs）作为样品分离器和信号放大器。该生物传感器在磷酸盐缓冲盐水（PBS）和鼻咽样品中进行了测试。结果表明，该传感器具有宽线性动态范围（0.01 ng/mL至1000 ng/mL）和低检测限（0.0072 ng/mL），实现了对刺突蛋白的稳定且灵敏的检测。因此，这种免疫传感方法提供了一种有前景的电化学测量工具，可为SARS-CoV-2的早期筛查和检测方法的合理优化提供思路。
