Xu Xiao, Ding Qian, Wang Xiu, Wang Ruijie, Ullah Farman, Gao Xiwu, Song Dunlun
Department of Entomology, China Agricultural University, Beijing, China.
Pest Manag Sci. 2022 Apr;78(4):1500-1507. doi: 10.1002/ps.6768. Epub 2022 Jan 18.
The peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is a major pest worldwide. The intensive use of insecticides has led to the development of resistance against neonicotinoid insecticides. The R81T mutation in the nicotinic acetylcholine receptor (nAChR) beta1 subunit is considered a crucial mechanism adaptation to neonicotinoid resistance in M. persicae and Aphis gossypii.
Resistance-related mutations (R81T and V101I) were detected in the imidacloprid-resistant M. persicae AH19 population. The V101I mutation is reported for the first time. The V101I and R81T mutations existed separately, indicating that the two mutations evolved independently. Imidacloprid resistance in the AH19 population was stable without insecticide exposure. Four mutant strains were selected from the population with stable resistance. The resistance of the AH19-T, AH19-I, and AH19-TI strains to imidacloprid, thiamethoxam, and dinotefuran was significantly increased compared with the AH19-W strain. Synergism bioassays showed that the inhibition of three detoxification enzymes did not affect imidacloprid resistance in the AH19-T and AH19-I strains. Expression of nAChR β1 subunits in the AH19-W, AH19-T, and AH19-I strains remained unchanged.
The V101I mutation is associated with neonicotinoid resistance in M. persicae. The resistance of the AH19-T and AH19-I strains to neonicotinoids appears to be mainly due to the R81T and V101I mutations, whereas these mutations, together with changes in the cytochrome P450 monooxygenases and nAChR expression may be responsible for the development of neonicotinoid resistance in the AH19-TI strain. © 2021 Society of Chemical Industry.
桃蚜（Myzus persicae (Sulzer)，半翅目：蚜科）是一种世界性的主要害虫。杀虫剂的大量使用导致了对新烟碱类杀虫剂的抗性发展。烟碱型乙酰胆碱受体（nAChR）β1亚基中的R81T突变被认为是桃蚜和棉蚜对新烟碱类抗性的关键适应机制。
在对吡虫啉具有抗性的桃蚜AH19种群中检测到了与抗性相关的突变（R81T和V101I）。V101I突变首次被报道。V101I和R81T突变分别存在，表明这两个突变是独立进化的。AH19种群对吡虫啉的抗性在无杀虫剂暴露的情况下是稳定的。从具有稳定抗性的种群中筛选出了四个突变株系。与AH19-W株系相比，AH19-T、AH19-I和AH19-TI株系对吡虫啉、噻虫嗪和呋虫胺的抗性显著增加。增效生物测定表明，三种解毒酶的抑制对AH19-T和AH19-I株系的吡虫啉抗性没有影响。AH19-W、AH19-T和AH19-I株系中nAChRβ1亚基的表达保持不变。
V101I突变与桃蚜对新烟碱类的抗性有关。AH19-T和AH19-I株系对新烟碱类的抗性似乎主要归因于R81T和V101I突变，而这些突变与细胞色素P450单加氧酶的变化和nAChR表达可能是AH19-TI株系新烟碱类抗性发展的原因。© 2021化学工业协会。
