Institute of Experimental Epileptology and Cognition Research, Life and Brain Center, University of Bonn Medical Center, Bonn, Germany.
International Max Planck Research School for Brain and Behavior, University of Bonn, Bonn, Germany.
PLoS Comput Biol. 2021 Dec 28;17(12):e1009725. doi: 10.1371/journal.pcbi.1009725. eCollection 2021 Dec.
The firing of neurons throughout the brain is determined by the precise relations between excitatory and inhibitory inputs, and disruption of their balance underlies many psychiatric diseases. Whether or not these inputs covary over time or between repeated stimuli remains unclear due to the lack of experimental methods for measuring both inputs simultaneously. We developed a new analytical framework for instantaneous and simultaneous measurements of both the excitatory and inhibitory neuronal inputs during a single trial under current clamp recording. This can be achieved by injecting a current composed of two high frequency sinusoidal components followed by analytical extraction of the conductances. We demonstrate the ability of this method to measure both inputs in a single trial under realistic recording constraints and from morphologically realistic CA1 pyramidal model cells. Future experimental implementation of our new method will facilitate the understanding of fundamental questions about the health and disease of the nervous system.
大脑中神经元的放电由兴奋性和抑制性输入之间的精确关系决定，而它们之间平衡的破坏是许多精神疾病的基础。由于缺乏同时测量这两种输入的实验方法，因此尚不清楚这些输入是否随时间或重复刺激而变化。我们开发了一种新的分析框架，用于在电流箝位记录下单次试验中同时即时测量兴奋性和抑制性神经元输入。这可以通过注入由两个高频正弦波分量组成的电流来实现，然后通过分析提取电导率。我们证明了该方法在现实记录约束和形态上逼真的 CA1 锥体模型细胞下单次试验中测量这两种输入的能力。我们新方法的未来实验实现将有助于理解有关神经系统健康和疾病的基本问题。
