Fakeeh College for Medical Sciences, Jeddah, Saudi Arabia.
Faculty of Medicine, Cairo University, Cairo, Egypt.
Nutr Cancer. 2022;74(7):2657-2670. doi: 10.1080/01635581.2021.2019794. Epub 2021 Dec 28.
The present study intended to compare the antioxidant, anti-lipid peroxidation, and anti-inflammatory potentials of (NS) and on 5-FU-induced liver damage in rats. 48 rats were divided into control, control group of the onion extract, control group of the NS extract, 5-FU-treated, concomitant NS-treated, and concomitant onion extract-treated. Liver sections were processed for histological analysis (light and electron microscopic examination). Liver enzymes (ALT, AST, and ALP), inflammatory markers (TNF-α and IL-1), antioxidant markers (SOD, GSH, and GSH/GSSG ratio), 4-HNE, NF-κB, and Nrf2 were evaluated. The 5-FU-treated group exhibited inflammation, congested hepatic sinusoid, and steatosis. Improvement with few pathological residues was seen in the concomitant extract-treated groups. The 5-FU-treated group showed higher liver enzymes. The enzymes decreased in the concomitantly treated groups. 5-FU induced liver damage through oxidative stress, inflammation, and lipid peroxidation. Concomitantly using NS and onion extracts resulted in a reduction in oxidative stress, lipid peroxidation, and inflammation. NS and onion extracts attenuated 5-FU-induced liver damage via antioxidative, anti-lipid peroxidative, and anti-inflammatory mechanisms. NS's role was exceptional when compared with onion extract.
本研究旨在比较（NS）和洋葱提取物对 5-FU 诱导的大鼠肝损伤的抗氧化、抗脂质过氧化和抗炎潜力。将 48 只大鼠分为对照组、洋葱提取物对照组、NS 提取物对照组、5-FU 处理组、同时给予 NS 处理组和同时给予洋葱提取物处理组。对肝组织进行处理以进行组织学分析（光镜和电镜检查）。评估肝酶（ALT、AST 和 ALP）、炎症标志物（TNF-α 和 IL-1）、抗氧化标志物（SOD、GSH 和 GSH/GSSG 比值）、4-HNE、NF-κB 和 Nrf2。5-FU 处理组表现出炎症、充血的肝窦和脂肪变性。同时给予提取物处理的各组中，观察到改善且残留的病理变化较少。5-FU 处理组的肝酶升高。同时给予提取物处理的各组中的酶降低。5-FU 通过氧化应激、炎症和脂质过氧化诱导肝损伤。同时使用 NS 和洋葱提取物可减少氧化应激、脂质过氧化和炎症。NS 和洋葱提取物通过抗氧化、抗脂质过氧化和抗炎机制减轻 5-FU 诱导的肝损伤。与洋葱提取物相比，NS 的作用更为突出。
