Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota, USA.
Microbiologyopen. 2021 Nov;10(6):e1256. doi: 10.1002/mbo3.1256.
Although Streptococcus agalactiae periprosthetic joint infection (PJI) is not as prevalent as staphylococcal PJI, invasive S. agalactiae infection is not uncommon. Here, RNA-seq was used to perform transcriptomic analysis of S. agalactiae PJI using fluid derived from sonication of explanted arthroplasties of subjects with S. agalactiae PJI, with results compared to those of S. agalactiae strain NEM316 grown in vitro. A total of 227 genes with outlier expression were found (164 upregulated and 63 downregulated) between PJI sonicate fluid and in vitro conditions. Functional enrichment analysis showed genes involved in mobilome and inorganic ion transport and metabolism to be most enriched. Genes involved in nickel, copper, and zinc transport, were upregulated. Among known virulence factors, cyl operon genes, encoding β-hemolysin/cytolysin, were consistently highly expressed in PJI versus in vitro. The data presented provide insight into S. agalactiae PJI pathogenesis and may be a resource for identification of novel PJI therapeutics or vaccines against invasive S. agalactiae infections.
虽然无乳链球菌假体周围关节感染 (PJI) 不如葡萄球菌 PJI 常见，但侵袭性无乳链球菌感染并不少见。在这里，使用 RNA-seq 对来自患有无乳链球菌 PJI 患者的关节置换物超声处理液中的无乳链球菌 PJI 进行了转录组分析，并将结果与体外培养的无乳链球菌 NEM316 菌株进行了比较。在 PJI 超声处理液和体外条件之间发现了 227 个具有异常表达的基因（164 个上调和 63 个下调）。功能富集分析显示，参与可移动体和无机离子运输和代谢的基因最为丰富。与镍、铜和锌运输相关的基因上调。在已知的毒力因子中，编码β-溶血性细胞素/细胞溶解素的 cyl 操纵子基因在 PJI 中与体外相比一致高表达。所提供的数据深入了解了无乳链球菌 PJI 的发病机制，可能为鉴定针对侵袭性无乳链球菌感染的新型 PJI 治疗药物或疫苗提供资源。
