Patterson S, Byrne J A, Lampert P W, Oldstone M B
Lab Invest. 1987 Jul;57(1):29-36.
The interactions between lymphocytic choriomeningitis virus-specific cloned cytotoxic T lymphocytes (CTL) and virus infected targets have been examined by electron microscopy. CTLs, which were readily differentiated from target cells by the presence of cytoplasmic granular inclusions, made intimate contact with infected cells. Some CTLs contacted infected cells via numerous interdigitating processes; others were observed thrusting finger-like protrusions deep into the target cell; some were seen with their plasma membranes lying closely opposed to that of the infected cell. The majority (55%) of bound CTLs had their Golgi apparatus oriented towards the target cell and 42% of bound CTL had granular inclusions in close proximity to the contact zone. Evidence is presented which suggests that the contents of the granular inclusions are released by CTLs in contact with infected cells. Granules appeared to be released close to the target cell rather than from random sites on the CTL surface. Examination of supernatants from effector-target cell incubation mixtures by negative staining revealed membranes bearing lesions with an internal diameter of approximately 15 nm.
通过电子显微镜检查了淋巴细胞性脉络丛脑膜炎病毒特异性克隆细胞毒性T淋巴细胞（CTL）与病毒感染靶细胞之间的相互作用。CTL通过细胞质颗粒内含物的存在很容易与靶细胞区分开来，它们与感染细胞进行密切接触。一些CTL通过许多相互交错的突起与感染细胞接触；观察到其他CTL将手指状突起深深插入靶细胞；有些CTL的质膜与感染细胞的质膜紧密相对。大多数（55%）结合的CTL其高尔基体朝向靶细胞，42%结合的CTL在接触区附近有颗粒内含物。有证据表明颗粒内含物的内容物由与感染细胞接触的CTL释放。颗粒似乎是在靠近靶细胞处释放，而不是从CTL表面的随机部位释放。通过负染色检查效应细胞 - 靶细胞孵育混合物的上清液，发现带有内径约15 nm损伤的膜。
