Korea Institute of Ocean Science and Technology, Busan, 49111, Republic of Korea.
Institute of Liberal Arts Education, Pukyong National University, Busan, 48547, Republic of Korea.
J Microbiol. 2022 Jan;60(1):11-17. doi: 10.1007/s12275-022-1468-6. Epub 2021 Dec 29.
A Gram-stain-negative, aerobic, rod-shaped (0.3-0.5 × 1.0-1.9 µm), non-motile marine bacterium designated as ALE3EI was isolated from a saline volcanic rock aquifer (lava sea-water) on Jeju Island, Republic of Korea. The 16S rRNA gene sequence analysis revealed that strain ALE3EI showed high similarity to 'Altibacter lentus' JLT2010 (97.2%), followed by Marixanthomonas ophiurae KMM 3046 (94.5%). Growth was observed at 10-41°C (optimum, 30°C), at pH 6.0-8.5 (optimum, pH 7.5) and at 0.5-8% (optimum, 4.0%) NaCl. The predominant cellular fatty acids were iso-C (23.5%), iso-C (10.2%), iso-C 3OH (10.5%), and iso-C 3OH (16.8%). The DNA G + C contents was 40.4 mol%. The major respiratory quinone was MK-6. The major polar lipids were determined to be phosphatidylethanolamine, two unidentified glycolipids, and two unidentified aminolipids. Several phenotypic characteristics such as production of acetoin, activities of arginine dihydrolase and acid phosphatase, and utilization pattern of carbon sources differentiate strain ALE3EI from 'A. lentus' JLT2010. Activities of the lipase, trypsin, α-chymotrypsin and gelatinase and utilization pattern of carbon sources differentiate strain ALE3EI from M. ophiurae KMM 3046. The genome of strain ALE3EI is 3.0 Mbp long and its ANI and AAI values against 'A. lentus' JLT2010 were 76.58 and 72.76, respectively, however, AAI values against members in other genera were lower than 72%. The phylogenomic tree inferred by PhyloPhlAn clearly differentiated the strain ALE3EI together with strain JLT2010 from other genera in the Falvobacteriaceae. This polyphasic taxonomic data indicates that strain ALE3EI should be identified as a novel species in the genus 'Altibacter', however, the name has not been validated. Therefore, the strain is classified as a novel genus and is proposed as Constantimarinum furrinae gen. nov., sp. nov. The type strain is ALE3EI (= KCCM 43303 = JCM 33022).
一株革兰氏阴性、需氧、杆状（0.3-0.5×1.0-1.9μm）、非运动性海洋细菌，被命名为 ALE3EI，从韩国济州岛的盐水火山岩含水层（海卤水）中分离得到。16S rRNA 基因序列分析表明，菌株 ALE3EI 与“Altibacter lentus”JLT2010（97.2%）高度相似，其次是 Marixanthomonas ophiurae KMM 3046（94.5%）。在 10-41°C（最佳，30°C）、pH 6.0-8.5（最佳，pH 7.5）和 0.5-8%（最佳，4.0%）NaCl 下可观察到生长。主要的细胞脂肪酸是 iso-C（23.5%）、iso-C（10.2%）、iso-C 3OH（10.5%）和 iso-C 3OH（16.8%）。DNA G+C 含量为 40.4mol%。主要的呼吸醌是 MK-6。主要的极性脂是磷脂酰乙醇胺、两种未鉴定的糖脂和两种未鉴定的胺脂。一些表型特征，如乙酰丁醇的产生、精氨酸二氢酶和酸性磷酸酶的活性以及碳源的利用模式，将菌株 ALE3EI 与“Altibacter lentus”JLT2010 区分开来。脂肪酶、胰蛋白酶、α-糜蛋白酶和明胶酶的活性以及碳源的利用模式将菌株 ALE3EI 与 Marixanthomonas ophiurae KMM 3046 区分开来。菌株 ALE3EI 的基因组长 3.0 Mbp，其与“Altibacter lentus”JLT2010 的 ANI 和 AAI 值分别为 76.58 和 72.76，然而，与其他属成员的 AAI 值低于 72%。通过 PhyloPhlAn 推断的系统发育树清楚地区分了菌株 ALE3EI 与 Falvobacteriaceae 中的其他属一起属于“Altibacter”属。这种多相分类学数据表明，菌株 ALE3EI 应被鉴定为“Altibacter”属的一个新种，但该名称尚未得到验证。因此，该菌株被分类为一个新属，并被提议为 Constantimarinum furrinae gen. nov.，sp. nov.。模式菌株为 ALE3EI（=KCCM 43303=JCM 33022）。
