Zhang Zenghu, Hu Zhenzhen, Tang Lili, Wang Zengmeng, Zhang Yongyu
Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People's Republic of China.
Institute of Marine Science and Technology, Shandong University, Qingdao, 266273, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Jul;112(7):1077-1085. doi: 10.1007/s10482-019-01239-x. Epub 2019 Feb 7.
A Gram-stain negative, aerobic, rod-shaped, and non-motile bacterium, designated strain CCMM003, was isolated from a culture of the green alga Ulva prolifera. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CCMM003 belongs to the family Flavobacteriaceae and exhibits a close relationship to Pseudozobellia thermophila DSM 19858 (92.5%). Optimal growth occurred in the presence of 4% (w/v) NaCl, at pH 7.0 and 30 °C. The polar lipids of strain CCMM003 consisted of phosphatidylethanolamine and six unidentified lipids. The predominant isoprenoid quinone was MK-6. The major fatty acids were iso-C, iso-C G, iso-C 3-OH and summed feature 3 (Cω7c and/or iso-C 2-OH). The DNA G + C content of strain CCMM003 calculated on the basis of the genome sequence was 41.2 mol% and the genome size was 5.9 Mbp. On the basis of data from this polyphasic study, strain CCMM003 is considered to represent a novel genus and species of the family Flavobacteriaceae, for which the name Ulvibacterium marinum gen. nov., sp. nov. is proposed. The type strain is CCMM003 (= MCCC 1K03244 =KCTC 52639).
从绿藻石莼培养物中分离出一株革兰氏染色阴性、需氧、杆状且无运动性的细菌，命名为CCMM003菌株。基于16S rRNA基因序列的系统发育分析表明，CCMM003菌株属于黄杆菌科，与嗜热假佐贝尔氏菌DSM 19858具有密切关系（92.5%）。在4%（w/v）NaCl、pH 7.0和30℃条件下生长最佳。CCMM003菌株的极性脂质由磷脂酰乙醇胺和六种未鉴定的脂质组成。主要类异戊二烯醌为MK-6。主要脂肪酸为异-C、异-C G、异-C 3-OH和总和特征3（Cω7c和/或异-C 2-OH）。根据基因组序列计算，CCMM003菌株的DNA G+C含量为41.2 mol%，基因组大小为5.9 Mbp。基于这项多相研究的数据，CCMM003菌株被认为代表黄杆菌科的一个新属和新种，为此提出了海洋石莼杆菌属（Ulvibacterium）新属，新种。模式菌株为CCMM003（=MCCC 1K03244=KCTC 52639）。
