PDE8B 和 DUOX2 的高表达与甲状腺癌转移能力相关。

High Expression of PDE8B and DUOX2 Associated with Ability of Metastasis in Thyroid Carcinoma.

机构信息

Department of Nuclear Medicine, The First People's Hospital of Lianyungang, The First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang City 222000, China.

出版信息

Comput Math Methods Med. 2021 Dec 20;2021:2362195. doi: 10.1155/2021/2362195. eCollection 2021.

DOI:10.1155/2021/2362195

PMID:34966441

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8712144/

Abstract

BACKGROUND

Hormone is an independent factor that induces differentiation of thyroid cancer (TC) cells. The thyroid-stimulating hormone (TSH) could promote the progression and invasion in TC cells. However, few genes related to hormone changes are studied in poorly differentiated metastatic TC. This study is aimed at constructing a gene set's coexpression correlation network and verifying the changes of some hub genes involved in regulating hormone levels.

METHODS

Microarray datasets of TC samples were obtained from public Gene Expression Omnibus (GEO) databases. R software and bioinformatics packages were utilized to identify the differentially expressed genes (DEGs), important gene module eigengenes, and hub genes. Subsequently, the Gene Ontology (GO) enrichment analysis was constructed to explore important biological processes that are associated with the mechanism of poorly differentiated TC. Finally, some hub gene expressions were validated through real-time PCR and immunoblotting.

RESULTS

Gene chip with category number GSE76039 was analyzed, and 1190 DEGs were screened with criteria of < 0.05 and ∣logfoldchange | >2. Our analysis showed that human dual oxidase 2 (DUOX2) and phosphodiesterase 8B (PDE8B) are the two important hub genes in a coexpression network. In addition, the validated experimental results showed that the expression levels of both DUOX2 and PDE8B were elevated in poorly differentiated metastatic TC tissues.

CONCLUSION

This study identified and validated that DUOX2 and PDE8B were significantly associated with the metastasis ability of thyroid carcinoma.

摘要

背景

激素是诱导甲状腺癌（TC）细胞分化的独立因素。促甲状腺激素（TSH）可促进 TC 细胞的进展和侵袭。然而，在低分化转移性 TC 中，研究与激素变化相关的基因较少。本研究旨在构建基因集的共表达相关网络，并验证参与调节激素水平的一些关键基因的变化。

方法

从公共基因表达综合数据库（GEO）中获取 TC 样本的微阵列数据集。利用 R 软件和生物信息学软件包来识别差异表达基因（DEGs）、重要基因模块特征基因和关键基因。随后，构建基因本体论（GO）富集分析，以探索与低分化 TC 机制相关的重要生物学过程。最后，通过实时 PCR 和免疫印迹验证了一些关键基因的表达。

结果

分析类别号为 GSE76039 的基因芯片，筛选出 1190 个差异表达基因，标准为 < 0.05 和∣logfoldchange | > 2。我们的分析表明，人双氧化酶 2（DUOX2）和磷酸二酯酶 8B（PDE8B）是共表达网络中的两个重要关键基因。此外，验证性实验结果表明，DUOX2 和 PDE8B 的表达水平在低分化转移性 TC 组织中均升高。