Department of Molecular Medicine and Genetics, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Asian Pac J Cancer Prev. 2021 Dec 1;22(12):4043-4049. doi: 10.31557/APJCP.2021.22.12.4043.
The prevalence of colorectal cancer (CRC) has been increasing in the world. There are different factors for colorectal cancer, and changes in the levels of gene expression such as miR-145-5p, DANCR and NRAS can be one of the factors.
The case-control study was performed on 40 CRC specimens and 40 adjacent healthy tissues. Fresh tumor and tumor-free adjacent tissue samples were obtained from patients who underwent the surgical operation as a conventional treatment procedure. After tumor resection, the specimens were immediately frozen in liquid nitrogen and stored at -80°C until further investigation. Cox regression was used to get the hazard ratios.
The mean ± SD age of the patients was 62.45± 12.89 years. Of these, 62.5% were males. The risk of death from CRC in patients with low miR-145-5p levels is about 10 times higher than the high expression levels (HR = 10.759, P = 0.009). High expression levels of NRAS can increase the risk of CRC death up to 4 times (HR = 4.12, P = 0.045). The study did not show a relationship between DANCR expression levels and death risk from CRC (HR = 1.582, P = 0.439).
These expression levels revealed that miRNA-145-5p and NRAS can be utilized as diagnostic biomarkers in colorectal cancer death. This may also introduce the microRNAs as colorectal cancer therapeutic targets.
结直肠癌（CRC）的患病率在全球范围内呈上升趋势。结直肠癌有不同的发病因素，miR-145-5p、DANCR 和 NRAS 等基因表达水平的变化可能是其中之一。
采用病例对照研究，对 40 例 CRC 标本和 40 例相邻的健康组织进行研究。对接受手术常规治疗的患者获取新鲜肿瘤和无肿瘤的相邻组织样本。肿瘤切除后，立即将标本置于液氮中冷冻，并储存在-80°C 直至进一步研究。采用 Cox 回归获得风险比。
患者的平均年龄±标准差为 62.45±12.89 岁。其中，62.5%为男性。miR-145-5p 低水平患者的 CRC 死亡风险约为高水平患者的 10 倍（HR=10.759，P=0.009）。NRAS 高水平表达可使 CRC 死亡风险增加约 4 倍（HR=4.12，P=0.045）。本研究未显示 DANCR 表达水平与 CRC 死亡风险之间存在相关性（HR=1.582，P=0.439）。
这些表达水平表明，miR-145-5p 和 NRAS 可作为结直肠癌死亡的诊断生物标志物。这也可能将 microRNAs 引入结直肠癌的治疗靶点。
