Department of Molecular Medicine and Genetics, Faculty of Medicine, Hamadan University of Medical Sciences, 6517838695, Hamadan, Iran.
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Street of Mahdieh, 6517838695, Hamadan, Iran.
Mol Biol Rep. 2021 Apr;48(4):3541-3547. doi: 10.1007/s11033-021-06373-2. Epub 2021 May 6.
Recent evidence reveals that miRNA sponges neutralize miRNAs activity by binding to miRNAs and sequester them from their relevant targets to regulate expression. The detailed mechanisms of sponge RNAs in colorectal cancer remain to be exactly determined. In this study DANCR, miR-145-5p, NRAS axis was evaluated and the diagnostic value of these targets was assessed in colorectal cancer patients. A case-control study was carried out on 40 samples of tumor tissues and 40 adjacent tissues. Total RNA was extracted, and then, the expression level of DANCR, miR-145-5p and NRAS was evaluated using qRT-PCR. In addition, the sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Our results revealed that the expression level of DANCR was significantly upregulated in colorectal cancer tissues (p < 0.001). It was demonstrated that DANCR could regulate NRAS expression by sponging miR-145-5 in colorectal cancer patients. Furthermore, the mean expression of miR-145-5p (p < 0.001) and NRAS (p < 0.001) was significantly different between tumor and normal tissue. A significant correlation was observed between DANCR and miR-145-5p (p = 0.001), and also between miR-145-5p and NRAS (p < 0.001). Sensitivity and specificity value for DANCR, miR-145-5p and NRAS were (0.875 and 0.725), (0.875 and 0.745), and (0.877 and 0.694), respectively. According to the values of sensitivities and specificity of DANCR, miR-145-5p and NRAS, confirmed with ROC curve analysis, these biomarkers may be useful in the screening and differentiating between tumor and control sample in colorectal neoplasm.
最近的证据表明，miRNA 海绵通过与 miRNA 结合并将其与相关靶标隔离来中和 miRNA 的活性，从而调节表达。海绵 RNA 在结直肠癌中的详细机制仍需进一步确定。在这项研究中，评估了 DANCR、miR-145-5p、NRAS 轴，并评估了这些靶点在结直肠癌患者中的诊断价值。对 40 例肿瘤组织和 40 例相邻组织进行了病例对照研究。提取总 RNA，然后使用 qRT-PCR 评估 DANCR、miR-145-5p 和 NRAS 的表达水平。此外，通过接收者操作特征 (ROC) 曲线分析评估这些标志物的敏感性和特异性。我们的结果表明，DANCR 在结直肠癌组织中的表达水平显著上调（p<0.001）。结果表明，DANCR 可以通过海绵 miR-145-5 调节结直肠癌患者的 NRAS 表达。此外，miR-145-5p（p<0.001）和 NRAS（p<0.001）的平均表达在肿瘤组织和正常组织之间有显著差异。DANCR 与 miR-145-5p 之间存在显著相关性（p=0.001），miR-145-5p 与 NRAS 之间也存在显著相关性（p<0.001）。DANCR、miR-145-5p 和 NRAS 的灵敏度和特异性值分别为（0.875 和 0.725）、（0.875 和 0.745）和（0.877 和 0.694）。根据 ROC 曲线分析确认的 DANCR、miR-145-5p 和 NRAS 的灵敏度和特异性值，这些生物标志物可能有助于结直肠肿瘤的筛查和区分肿瘤样本和对照样本。
