Williams D G, Brennan F M, Stocks M R, Maini R N
Clin Exp Immunol. 1986 Sep;65(3):506-12.
The prevalence and diversity of anti-Sm antibodies (which are a marker of human lupus) were investigated in MRL/MP-lpr/lpr mice. The low prevalence of anti-Sm reported by other workers was confirmed. Anti-Sm antibodies bound to either the 28 kD or both 28 and 16 kD polypeptides on immunoblots. Antibodies to the 16 kD polypeptide, detected on spectrotyping blots, were not detected on immunoblots probed with sera from three mice, suggesting that these antibodies were binding only to an additional conformational epitope. Isoelectric focusing of the sera showed that the anti-Sm antibodies were restricted in clonal diversity and in some cases resembled the pattern of a monoclonal antibody. A longitudinal increase in antibody titre was correlated in some mice with an increase in the diversity of clones expressing anti-Sm antibody. Anti-nRNP antibodies were also detected in the mouse serum but had a lower prevalence than anti-Sm antibodies.
在MRL/MP-lpr/lpr小鼠中研究了抗Sm抗体（人类狼疮的标志物）的患病率和多样性。其他研究人员报告的抗Sm低患病率得到了证实。在免疫印迹中，抗Sm抗体与28 kD多肽或28 kD和16 kD多肽两者结合。在光谱分型印迹上检测到的针对16 kD多肽的抗体，在用三只小鼠的血清进行免疫印迹检测时未检测到，这表明这些抗体仅与另外的构象表位结合。血清的等电聚焦显示，抗Sm抗体在克隆多样性方面受到限制，在某些情况下类似于单克隆抗体的模式。在一些小鼠中，抗体滴度的纵向增加与表达抗Sm抗体的克隆多样性增加相关。在小鼠血清中也检测到了抗nRNP抗体，但其患病率低于抗Sm抗体。
