Shyamli P Sushree, Pradhan Seema, Panda Mitrabinda, Parida Ajay
Institute of Life Sciences, An Autonomous Institute Under Department of Biotechnology Government of India, NALCO Square, Bhubaneswar, India.
Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.
Front Plant Sci. 2021 Dec 14;12:766999. doi: 10.3389/fpls.2021.766999. eCollection 2021.
Abiotic stresses, especially drought stress, are responsible for heavy losses in productivity, which in turn poses an imminent threat for future food security. Understanding plants' response to abiotic stress at the molecular level is crucially important for mitigating the impacts of climate change. is an important multipurpose plant with medicinal and nutritional properties and with an ability to grow in low water conditions, which makes the species an ideal candidate to study the regulatory mechanisms that modulate drought tolerance and its possible use in agroforestry system. In the present communication, we report whole-genome sequencing (WGS) of this species and assemble about 90% of the genome of var. Bhagya into 915 contigs with a N50 value of 4.7 Mb and predicted 32,062 putative protein-coding genes. After annotating the genome, we have chosen to study the heat shock transcription factor (HSF) family of genes to analyze their role in drought tolerance in . We predicted a total of 21 HSFs in the genome and carried out phylogenetic analyses, motif identification, analysis of gene duplication events, and differential expression of the HSF-coding genes in . Our analysis reveals that members of the HSF family have an important role in the plant's response to abiotic stress and are viable candidates for further characterization.
非生物胁迫，尤其是干旱胁迫，是导致生产力严重损失的原因，这反过来又对未来的粮食安全构成了紧迫威胁。在分子水平上了解植物对非生物胁迫的反应对于减轻气候变化的影响至关重要。[物种名称]是一种具有药用和营养特性的重要多用途植物，并且能够在低水条件下生长，这使得该物种成为研究调节耐旱性的调控机制及其在农林业系统中可能用途的理想候选者。在本通讯中，我们报告了该物种的全基因组测序（WGS），并将[品种名称]约90%的基因组组装成915个重叠群，N50值为4.7 Mb，并预测了32062个推定的蛋白质编码基因。在对基因组进行注释后，我们选择研究热休克转录因子（HSF）基因家族，以分析它们在[物种名称]耐旱性中的作用。我们在[物种名称]基因组中总共预测了21个HSF，并进行了系统发育分析、基序鉴定、基因复制事件分析以及[物种名称]中HSF编码基因的差异表达分析。我们的分析表明，HSF家族成员在植物对非生物胁迫的反应中具有重要作用，并且是进一步表征的可行候选者。
