Incidence and genetic diversity of multi-drug resistant Listeria monocytogenes isolates recovered from fruits and vegetables in the Eastern Cape Province, South Africa.

We investigated the prevalence, genetic diversity and antibiogram profiles of Listeria monocytogenes (Lm) recovered from fruits and vegetables sourced from three District Municipalities in the Eastern Cape Province, South Africa after the recent listeriosis outbreak in the country. The procedure outlined by the International Organization for Standardization EN ISO 11290:2017 Parts 1 and 2 was adopted for the isolation of Lm from 140 vegetable samples. Molecular detection of the pathogen and the presence of 10 virulence-associated markers were assessed. Lm was detected in 42.86% of all the vegetable samples tested. Highest prevalence was recorded in tomato (65.52%) followed by spinach (56.67%), cabbage (38.10%), apple (36.84%), mushroom (29.41%) and carrot (10%). The virulence determinants including the inlA, inlC, prfA and plcA, hly, plcB genes were detected in all Lm isolates whereas, inlJ (88.35%), inlB (86.41%), mpl (92.23%) and actA (84.55%) respectively. High susceptibility (> 50) was observed to all antibiotics tested except for sulfamethoxazole (17.48%), streptomycin (38.84%), amoxicillin (41.75%) and erythromycin (43.69%). However, high resistance against sulfamethoxazole (80.58%), amoxicillin (58.25%) and erythromycin (49.52%) were observed. About 85.44% of Lm isolates showed multidrug-resistance phenotypes against the test antibiotics. Furthermore, twenty (20) resistance genes encoding tetracyclines, sulphonamides, phenicols, aminoglycosides, β-lactamases, and variants of the extended-spectrum of β-lactamases (ESBLs) resistance were detected among the Lm isolates. The sul2 (90.81), tetM (68.42%) sul1 (45.98%) were more prevalent among the resistant strains. The dendrogram signatures generating seven clades is an indication of the high genetic diversity among the isolates. We conclude that the presence of Lm in fruits and vegetables is a potential threat to the consumers and a potential public health hazard, particularly to the high-risk group of the population.