Ganić Tea, Vuletić Stefana, Nikolić Biljana, Stevanović Magdalena, Kuzmanović Maja, Kekić Dušan, Đurović Saša, Cvetković Stefana, Mitić-Ćulafić Dragana
Faculty of Biology, University of Belgrade, Belgrade, Serbia.
Group for Biomedical Engineering and Nanobiotechnology, Institute of Technical Sciences of SASA, Belgrade, Serbia.
Front Microbiol. 2022 Oct 10;13:989667. doi: 10.3389/fmicb.2022.989667. eCollection 2022.
is an emerging nosocomial pathogen resistant to a wide spectrum of antibiotics, with great potential to form a biofilm, which further aggravates treatment of infections caused by it. Therefore, searching for new potent agents that are efficient against seems to be a necessity. One of them, which has already been proven to possess a wide spectrum of biological activities, including antimicrobial effect, is cinnamon essential oil. Still, further increase of antibacterial efficacy and improvement of bioavailability of cinnamon oil is possible by emulsification process. The aim of this study was comparative analysis of cinnamon essential oil and its emulsion against biofilm forming clinical isolates. Furthermore, the investigation of toxicological aspects of possible applications of essential oil and emulsion was done as well. Gas chromatography-mass spectrometry of essential oil indicated -cinnamaldehyde as the most abundant component. The cinnamon emulsion was synthesized from cinnamon essential oil by combining modified low- and high- energy methods. Synthesized emulsion was characterized with Fourier-transform infrared spectroscopy and photon correlation spectroscopy. Both substances exhibited significant antibacterial (minimal inhibitory concentrations in the range 0.125-0.5 mg/ml) and antibiofilm effects (inhibitions of formation and reduction of pre-formed biofilm were 47-81 and 30-62%, respectively). Compared to essential oil, the efficacy of emulsion was even stronger considering the small share of pure oil (20%) in the emulsion. The result of biofilm eradication assay was confirmed by scanning electron microscopy. Even though the cytotoxicity was high especially for the emulsion, genotoxicity was not determined. In conclusion, strong antibacterial/antibiofilm effect against of the cinnamon essential oil and the fact that emulsification even potentiated the activity, seems to be of great significance. Observed cytotoxicity implicated that further analysis is needed in order to clearly determine active principles being responsible for obtained antibacterial/antibiofilm and cytotoxic properties.
是一种新出现的医院病原体，对多种抗生素耐药，具有形成生物膜的巨大潜力，这进一步加剧了由其引起的感染的治疗难度。因此，寻找对其有效的新型强效药物似乎很有必要。肉桂精油就是其中之一，它已被证明具有广泛的生物活性，包括抗菌作用。不过，通过乳化过程可以进一步提高肉桂油的抗菌效力并改善其生物利用度。本研究的目的是比较肉桂精油及其乳液对形成生物膜的临床分离株的作用。此外，还对精油和乳液可能应用的毒理学方面进行了研究。精油的气相色谱 - 质谱分析表明反式肉桂醛是最主要的成分。肉桂乳液是通过结合改良的低能和高能方法由肉桂精油合成的。合成的乳液用傅里叶变换红外光谱和光子相关光谱进行了表征。两种物质都表现出显著的抗菌作用（最低抑菌浓度在0.125 - 0.5毫克/毫升范围内）和抗生物膜作用（生物膜形成抑制率和预形成生物膜减少率分别为47 - 81%和30 - 62%）。与精油相比，考虑到乳液中纯油的比例较小（20%），乳液的效力更强。生物膜根除试验的结果通过扫描电子显微镜得到了证实。尽管细胞毒性很高，尤其是乳液的细胞毒性，但未检测到遗传毒性。总之，肉桂精油对具有强大的抗菌/抗生物膜作用，并且乳化甚至增强了这种活性，这似乎具有重要意义。观察到的细胞毒性表明需要进一步分析，以便明确确定导致所获得的抗菌/抗生物膜和细胞毒性特性的活性成分。
