Kang Min-Gyun, Khan Fazlurrahman, Tabassum Nazia, Cho Kyung-Jin, Jo Du-Min, Kim Young-Mog
Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea.
Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea.
ACS Omega. 2023 Mar 7;8(11):9873-9888. doi: 10.1021/acsomega.2c06789. eCollection 2023 Mar 21.
The emergence of antibiotic resistance in microbial pathogens necessitates the development of alternative ways to combat the infections that arise. The current study used nanotechnology as an alternate technique to control virulence characteristics and biofilm development in and . Furthermore, based on the acceptance and biocompatibility of the probiotic bacteria, we chose a lactic acid bacteria (LAB) for synthesizing two types of metallic nanoparticles (NPs) in this study. Using molecular techniques, the LAB strain C1 was isolated from Kimchi food samples and identified as sp. strain C1. The prepared supernatant from strain C1 was used to produce gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). C1-AuNPs and C1-AgNPs were characterized physiochemically using a variety of instruments. C1-AuNPs and C1-AgNPs had spherical shapes and sizes of 100.54 ± 14.07 nm (AuNPs) and 129.51 ± 12.31 nm (AgNPs), respectively. C1-AuNPs and C1-AgNPs were discovered to have high zeta potentials of -23.29 ± 1.17 and -30.57 ± 0.29 mV, respectively. These nanoparticles have antibacterial properties against several bacterial pathogens. C1-AuNPs and C1-AgNPs significantly inhibited the initial stage biofilm formation and effectively eradicated established mature biofilms of and . Furthermore, when was treated with sub-MIC levels of C1-AuNPs and C1-AgNPs, their different virulence features were significantly reduced. Both NPs greatly inhibited the hemolytic activity of . The inhibition of and biofilms and virulence features by C1-AuNPs and C1-AgNPs can be regarded as viable therapeutic strategies for preventing infections caused by these bacteria.
微生物病原体中抗生素耐药性的出现使得有必要开发对抗由此引发感染的替代方法。当前研究将纳米技术用作一种替代技术，以控制[未提及具体细菌名称]的毒力特征和生物膜形成。此外，基于益生菌的可接受性和生物相容性，本研究选择了一种乳酸菌（LAB）来合成两种类型的金属纳米颗粒（NPs）。使用分子技术，从泡菜食品样本中分离出LAB菌株C1，并鉴定为[未提及具体菌种名称]菌株C1。用菌株C1制备的上清液用于生产金纳米颗粒（AuNPs）和银纳米颗粒（AgNPs）。使用多种仪器对C1-AuNPs和C1-AgNPs进行了物理化学表征。C1-AuNPs和C1-AgNPs呈球形，尺寸分别为100.54±14.07 nm（AuNPs）和129.51±12.31 nm（AgNPs）。发现C1-AuNPs和C1-AgNPs分别具有-23.29±1.17和-30.57±0.29 mV的高zeta电位。这些纳米颗粒对几种细菌病原体具有抗菌特性。C1-AuNPs和C1-AgNPs显著抑制了生物膜形成的初始阶段，并有效根除了[未提及具体细菌名称]已形成的成熟生物膜。此外，当[未提及具体细菌名称]用亚最小抑菌浓度水平的C1-AuNPs和C1-AgNPs处理时，其不同的毒力特征显著降低。两种纳米颗粒都极大地抑制了[未提及具体细菌名称]的溶血活性。C1-AuNPs和C1-AgNPs对[未提及具体细菌名称]生物膜和毒力特征的抑制可被视为预防这些细菌引起感染的可行治疗策略。
