莪术根茎和薤白抑制一氧化氮产生和大麻素受体 2 下调。
Curcumae Longae Rhizoma and Saussureae Radix Inhibit Nitric Oxide Production and Cannabinoid Receptor 2 Down-regulation.
机构信息
Laboratory of Nutri-Pharmacotherapeutics Management, School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan;
Laboratory of Pharmacognocy, School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan;
出版信息
In Vivo. 2022 Jan-Feb;36(1):227-232. doi: 10.21873/invivo.12695.
BACKGROUND/AIM: The cannabinoid 2 (CB2) receptor is an important regulator of immunoinflammatory responses. Crude drugs commonly used in Japanese traditional Kampo medicine have displayed anti-inflammatory effects; however, few studies have reported that these effects are mediated via CB2 receptor signaling. Therefore, this study aimed to elucidate CB2 receptor-related anti-inflammatory regulation in crude drugs.
MATERIALS AND METHODS
The ethanol extracts of 34 crude drugs listed in the Japanese Pharmacopeia were tested, and the inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production were evaluated in murine macrophage RAW 264 cells.
RESULTS
The extracts of Curcumae Longae Rhizoma (dried rhizome of Curcuma longa) and Saussureae Radix (dried root of Saussurea lappa) significantly inhibited NO production and attenuated the LPS-induced decrease in CB2 receptor mRNA expression.
CONCLUSION
Curcumae Longae Rhizoma and Saussureae Radix can modulate the CB2-receptor-related anti-inflammatory regulation in macrophages.
背景/目的：大麻素 2（CB2）受体是免疫炎症反应的重要调节因子。日本传统汉方医学中常用的粗药具有抗炎作用；然而，很少有研究报道这些作用是通过 CB2 受体信号转导介导的。因此，本研究旨在阐明粗药中与 CB2 受体相关的抗炎调节作用。
材料和方法
对日本药典中列出的 34 种粗药的乙醇提取物进行了测试，并在小鼠巨噬细胞 RAW 264 细胞中评估了它们对脂多糖（LPS）诱导的一氧化氮（NO）产生的抑制作用。
结果
姜黄（姜黄的干燥根茎）和雪莲花（雪莲花的干燥根）的提取物显著抑制了 NO 的产生，并减轻了 LPS 诱导的 CB2 受体 mRNA 表达降低。
结论
姜黄和雪莲花可以调节巨噬细胞中与 CB2 受体相关的抗炎调节作用。
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