Wu Dong-Xue, Hou Ning, Li Jing, Liu Min, Zhang Yan-Ling, Qiao Yan-Jiang
State Administration of Traditional Chinese Medicine,Research Center of Traditional Chinese Medicine-Information Engineering,Beijing University of Chinese Medicine Beijing 100102,China.
Zhongguo Zhong Yao Za Zhi. 2019 Jan;44(2):229-234. doi: 10.19540/j.cnki.cjcmm.20180903.003.
Curcumae Longae Rhizoma,Curcumae Radix and Curcumae Rhizoma are different medicinal parts of the same plant.Nevertheless,they are different in medicinal effects due to the different Chinese herbal nature. In this study,traditional Chinese medicines database( TCMD2009),traditional Chinese medicine system( TCMSP),and Ch EMBL database were retrieved to screen the active components and targets,and construct the target PPI network. By a graph theoretic clustering algorithm identifying protein complex algorithm( IPCA),the protein modules were identified and analyzed by gene ontology( GO) enrichment. A comparative study of Curcumae Longae Rhizoma and Curcumae Radix illustrate that Curcumae Longae Rhizoma regulates blood coagulation through P2 RY12,GNG2 and other genes to exert the analgesic effect. Curcumae Radix regulates lipid metabolism,plasma lipoprotein particle levels,platelet activation,response to oxidative stress,apoptotic process through LDLR,APOB,PRKCA,SOD1,TP53 and other genes to perform a function in clearing the heart and cooling the blood. A comparative study of Curcumae Radix and Curcumae Rhizoma demonstrate that Curcumae Rhizoma on regulates the nervous system by GRIA2,GRIA4 and other genes to exert blood-breaking effect; Curcumae Radix regulates lipid metabolism,plasma lipoprotein particle levels,platelet activation,response to oxidative stress,apoptotic process by genes such as CALM1,LPL,APOB,SOD1 and TP53 to play the role of clearing heart and cooling blood. Cluster analysis of the protein interaction network of the nature combination is helpful to explain the intrinsic link between the nature combination and efficacy.
姜黄、郁金和莪术是同一植物的不同药用部位。然而，由于中药药性不同，它们的药用功效也有所差异。本研究通过检索中药数据库（TCMD2009）、中药系统药理学数据库（TCMSP）和ChEMBL数据库筛选活性成分及靶点，构建靶点蛋白-蛋白相互作用（PPI）网络。运用图论聚类算法中的识别蛋白质复合体算法（IPCA）识别蛋白质模块，并通过基因本体（GO）富集分析。姜黄与郁金的对比研究表明，姜黄通过P2RY12、GNG2等基因调节凝血以发挥镇痛作用。郁金通过LDLR、APOB、PRKCA、SOD1、TP53等基因调节脂质代谢、血浆脂蛋白颗粒水平、血小板活化、氧化应激反应、凋亡过程，从而发挥清心凉血的作用。莪术与郁金的对比研究表明，莪术通过GRIA2、GRIA4等基因调节神经系统以发挥破血作用；郁金通过CALM1、LPL、APOB、SOD1、TP53等基因调节脂质代谢、血浆脂蛋白颗粒水平、血小板活化、氧化应激反应、凋亡过程，发挥清心凉血的作用。对中药药性组合的蛋白质相互作用网络进行聚类分析，有助于阐释药性组合与功效之间的内在联系。
