Yapryntseva Maria A, Zhivotovsky Boris, Gogvadze Vladimir
Faculty of Basic Medicine, Lomonosov Moscow State University, Moscow, Russia.
Karolinska Institutet, Institute of Environmental Medicine, Stockholm, Sweden.
Methods Mol Biol. 2022;2445:227-239. doi: 10.1007/978-1-0716-2071-7_14.
Mitophagy, a process of selective elimination of mitochondria by autophagy, is a mechanism of mitochondrial quality control that maintains mitochondrial network functionality. The elimination of damaged mitochondria through autophagy requires two steps: induction of general autophagy and priming of damaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.
线粒体自噬是一种通过自噬选择性清除线粒体的过程，是维持线粒体网络功能的线粒体质量控制机制。通过自噬清除受损线粒体需要两个步骤：诱导一般自噬和使受损线粒体启动以进行选择性自噬识别。线粒体自噬功能障碍与多种病理状况相关；因此，清除功能失调甚至有害的线粒体对细胞生理至关重要。在这里，我们描述了可应用于线粒体自噬研究的方法。
