School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
J Biomed Nanotechnol. 2021 Dec 1;17(12):2485-2494. doi: 10.1166/jbn.2021.3200.
Chinese medicine is a treasure of the Chinese nation, and charcoal drugs are a class of medicine with distinctive characteristics. (SRC) could be a sort of calcined herb medicate that has been utilized in traditional Chinese medicine (TCM) clinics to treat hypersensitivities. However, to date, the function of the carbonized part and action mechanisms of SRCs have not been elucidated. In this study, novel water-soluble carbon dots (CDs, named SRC-CDs) ranging from 2 to 9 nm were observed and separated from aqueous extracts of SRC. These SRC-CDs were characterized using transmission electron microscopy (TEM) and high-resolution TEM, as well as Fourier transform infrared, ultraviolet-visible, and fluorescence spectroscopy, to determine particle size, morphology, chemical structure, and optical properties. Then, the antiallergic efficacy of the SRC-CDs was studied in a C48/80-induced RBL-2H3 cell model, in which remarkable antiallergic effects were revealed. These results will provide new solution directions and technical methods for follow-up research of charcoal drugs and new understanding of potential biomedical applications of CDs.
中药是中华民族的瑰宝，炭药是一类特色鲜明的药物。（SRC）可能是一种煅烧草药，已在中医（TCM）诊所中用于治疗过敏。然而，迄今为止，碳化部分的功能和 SRC 的作用机制尚未阐明。在这项研究中，从 SRC 的水提物中观察到并分离出新型水溶性碳点（CDs，命名为 SRC-CDs），其粒径为 2 至 9nm。使用透射电子显微镜（TEM）和高分辨率 TEM 以及傅里叶变换红外、紫外-可见和荧光光谱对这些 SRC-CDs 进行了表征，以确定粒径、形态、化学结构和光学性质。然后，在 C48/80 诱导的 RBL-2H3 细胞模型中研究了 SRC-CDs 的抗过敏功效，结果显示出显著的抗过敏作用。这些结果将为炭药的后续研究提供新的解决方案方向和技术方法，并为 CDs 的潜在生物医学应用提供新的认识。
