College of Animal Science and Technology, Henan university of animal husbandry and Economy, Zhengzhou 450046, China.
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Ecotoxicol Environ Saf. 2023 Mar 1;252:114609. doi: 10.1016/j.ecoenv.2023.114609. Epub 2023 Feb 3.
Interactions between host and pathogen are involving various dynamic changes in transcript expression and critical for understanding host immunity against infections and its associated pathogenesis. Herein, we established a model of channel catfish infected with Aeromonas veronii. The infected fish had prominent body surface bleeding, and the spleen showed hyperemia and swelling. Then, the spleen of channel catfish infected with A. veronii was analyzed by dual RNA sequencing (RNA-seq), and the transcriptome data were compared with uninfected channel catfish spleen or bacteria cultured in vitro. The transcript expression profile of pathogen-host interaction between A. veronii and channel catfish was successfully studied. During infection, the host was enriched for multiple immune-related signaling pathways, such as the Toll-like receptor signaling pathway, Cytokine-cytokine receptor interaction, and T cell receptor signaling pathway; and significantly upregulated for many innate immune-related genes, including IL-8. At the same time, we found that A. veronii mainly harmed the host spleen through hemolysin. Our current findings are of great significance in clarifying the pathogenesis of channel catfish induced by A. veronii and provide gene targets for developing preventive measures.
宿主与病原体之间的相互作用涉及转录表达的各种动态变化，对于理解宿主对感染的免疫及其相关发病机制至关重要。在此，我们建立了感染维氏气单胞菌的斑点叉尾鮰模型。感染鱼体表有明显出血，脾脏充血肿胀。然后，通过双 RNA 测序（RNA-seq）分析感染维氏气单胞菌的斑点叉尾鮰脾脏，并将转录组数据与未感染斑点叉尾鮰脾脏或体外培养的细菌进行比较。成功研究了维氏气单胞菌与斑点叉尾鮰之间的病原体-宿主相互作用的转录表达谱。在感染过程中，宿主富集了多种免疫相关信号通路，如 Toll 样受体信号通路、细胞因子-细胞因子受体相互作用和 T 细胞受体信号通路；并显著上调了许多先天免疫相关基因，包括 IL-8。同时，我们发现维氏气单胞菌主要通过溶血素对宿主脾脏造成损害。我们目前的研究结果对于阐明维氏气单胞菌诱导的斑点叉尾鮰发病机制具有重要意义，并为开发预防措施提供了基因靶点。
