Lyerly H K, Cohen O J, Weinhold K J
AIDS Res Hum Retroviruses. 1987 Spring;3(1):87-94. doi: 10.1089/aid.1987.3.87.
Tetanus toxoid (TT) reactive CD4+ cells were infected with HTLV-IIIB and exposed to TT at various times throughout a 7-day interval. Acute infection per se failed to produce overt cytopathology. However, exposure of infected cells to TT resulted in a rapid loss of cell viability, an increase in viral p24 expression, and a decline in T-cell blastogenesis. To determine whether HIV infection of antigen presenting cells (APC) could impact on T-cell activation, virus infected APC were utilized to present TT to responsive CD4+ cells. Use of infected APC produced effects similar to antigen stimulation of infected T-cells. These results suggest that the conditions of antigen presentation during T-cell activation may provide an excellent opportunity for virus transmission which may produce maximal immune dysfunction. However, preincubating antigen specific T-cells with the virostatic agent 3'-azido-3'-deoxythymidine (AZT) could prevent most of these effects.
破伤风类毒素（TT）反应性CD4+细胞感染了HTLV-IIIB，并在7天的间隔内不同时间点暴露于TT。单纯的急性感染未能产生明显的细胞病理学变化。然而，将感染细胞暴露于TT会导致细胞活力迅速丧失、病毒p24表达增加以及T细胞母细胞化下降。为了确定抗原呈递细胞（APC）的HIV感染是否会影响T细胞活化，利用感染病毒的APC将TT呈递给反应性CD4+细胞。使用感染的APC产生的效果类似于感染T细胞的抗原刺激。这些结果表明，T细胞活化期间的抗原呈递条件可能为病毒传播提供绝佳机会，这可能导致最大程度的免疫功能障碍。然而，用抗病毒药物3'-叠氮-3'-脱氧胸苷（AZT）预孵育抗原特异性T细胞可以预防大多数这些效应。
