Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, China.
Departments of Diagnostic Radiology, Surgery, Chemical and Biomolecular Engineering, and Biomedical Engineering, Yong Loo Lin School of Medicine and Faculty of Engineering, National University of Singapore, Singapore, 119074, Singapore.
Nanotheranostics. 2022 Jan 1;6(1):91-102. doi: 10.7150/ntno.63124. eCollection 2022.
Biomedical luminescence imaging in the near-infrared (NIR, 700-1700 nm) region has shown great potential in visualizing biological processes and pathological conditions at cellular and animal levels, owing to the reduced tissue absorption and scattering compared to light in the visible (400-700 nm) region. To overcome the background interference and signal attenuation during intensity-based luminescence imaging, lifetime imaging has demonstrated a reliable imaging modality complementary to intensity measurement. Several selective or environment-responsive probes have been successfully developed for luminescence lifetime imaging and multiplex detection. This review summarizes recent advances in the application of luminescence lifetime imaging at cellular and animal levels in NIR-I and NIR-II regions. Finally, the challenges and further directions of luminescence lifetime imaging are also discussed.
生物医学近红外（NIR，700-1700nm）区域的生物发光成像是可视化细胞和动物水平的生物过程和病理状况的有力工具，这是因为与可见光（400-700nm）区域相比，NIR 区域的光在组织中的吸收和散射减少了。为了克服基于强度的发光成像过程中的背景干扰和信号衰减，寿命成像已被证明是一种与强度测量互补的可靠成像方式。已经成功开发了几种选择性或环境响应探针，用于发光寿命成像和多重检测。本文综述了 NIR-I 和 NIR-II 区域在细胞和动物水平上应用发光寿命成像的最新进展。最后，还讨论了发光寿命成像的挑战和进一步的发展方向。
