Liu Zhengjie, Xu Dongxin, Fang Jiaru, Xia Qijian, Zhong Wenxi, Li Hongbo, Huang Zhanyun, Cao Nan, Liu Xingxing, Chen Hui-Jiuan, Hu Ning
State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, China.
Laboratory Teaching Center of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China.
Front Bioeng Biotechnol. 2021 Dec 15;9:799312. doi: 10.3389/fbioe.2021.799312. eCollection 2021.
The electrophysiological signal can reflect the basic activity of cardiomyocytes, which is often used to study the working mechanism of heart. Intracellular recording is a powerful technique for studying transmembrane potential, proving a favorable strategy for electrophysiological research. To obtain high-quality and high-throughput intracellular electrical signals, an integrated electrical signal recording and electrical pulse regulating system based on nanopatterned microelectrode array (NPMEA) is developed in this work. Due to the large impedance of the electrode, a high-input impedance preamplifier is required. The high-frequency noise of the circuit and the baseline drift of the sensor are suppressed by a band-pass filter. After amplifying the signal, the data acquisition card (DAQ) is used to collect the signal. Meanwhile, the DAQ is utilized to generate pulses, achieving the electroporation of cells by NPMEA. Each channel uses a voltage follower to improve the pulse driving ability and isolates each electrode. The corresponding recording control software based on LabVIEW is developed to control the DAQ to collect, display and record electrical signals, and generate pulses. This integrated system can achieve high-throughput detection of intracellular electrical signals and provide a reliable recording tool for cell electro-physiological investigation.
电生理信号能够反映心肌细胞的基本活动，常用于研究心脏的工作机制。细胞内记录是研究跨膜电位的一项强大技术，为电生理研究提供了一种良好的策略。为了获得高质量、高通量的细胞内电信号，本文开发了一种基于纳米图案微电极阵列（NPMEA）的集成电信号记录与电脉冲调节系统。由于电极阻抗较大，需要一个高输入阻抗前置放大器。通过带通滤波器抑制电路的高频噪声和传感器的基线漂移。信号放大后，使用数据采集卡（DAQ）采集信号。同时，利用DAQ产生脉冲，通过NPMEA实现细胞电穿孔。每个通道使用一个电压跟随器来提高脉冲驱动能力并隔离每个电极。开发了基于LabVIEW的相应记录控制软件，以控制DAQ采集、显示和记录电信号，并产生脉冲。该集成系统能够实现细胞内电信号的高通量检测，为细胞电生理研究提供可靠的记录工具。
